据不完全统计，目前全国有近200个课题组正利用秀丽线虫作为模式生物进行创新性的基础与应用研究。为了更好地服务于国内的线虫研究圈，合理缩减科研人员的实验时间，并加速实现实验员的研究成果，我们精心挑选了广受大家欢迎的syb突变株系列。这254个突变株完全属于上源生科的自主产权，并且已被客户在发表文章后贡献给了美国的CGC（Caenorhabditis Genetics Center）作为突变株资源。 为了方便大家直接订购，并避免在国际订购过程中可能遇到的各种运输和清关问题，您只需直接点击下表首列的“strain name”，即可查看详细的突变序列信息。欢迎您添加我们的微信号SYSK0591进行咨询和下单。
搜索：| Strain Name | Genotype | Description |
|---|---|---|
| PHX209 | R12G8.1(syb209)V | syb209 is null allele (1143bp deletion), delete the whole gene of R12G8.1 on N2 by CRISPR/Cas9 system.
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| PHX235 | nlp-42(syb235)V | syb235 is null allele (2317bp deletion), delete the whole gene of nlp-42 on N2 by CRISPR/Cas9 system.
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| PHX267 | ikb-1(syb267)I | syb267 is a mcherry knock-in inserted before the termination codon of endogenous ikb-1 gene on N2 by CRISPR/Cas9 system.
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| PHX293 | nas-38(syb293)X | syb293 is deletion allele (443bp deletion), delete part of the gene of nas-38 on N2 by CRISPR/Cas9 system.. |
| PHX362 | vglu-2(syb362)III | syb362 is a GASGASGAS-GFP(pdd282) knock-in inserted before the termination codon of endogenous vglu-2 gene on N2 by CRISPR/Cas9 system. |
| PHX486 | C17D12.7(syb486)I | syb486 is a GFP-GGGGT knock-in inserted after the initiation codon of endogenous C17D12.7 gene on N2 by CRISPR/Cas9 system. |
| PHX509 | nhr-67(syb509) | syb509 is a GASGASGAS-GFP-3xFlag knock-in inserted before the termination codon of endogenous nhr-67 gene on N2 by CRISPR/Cas9 system. |
| PHX530 | nlp-11(syb530) | syb530 is null allele (2042bp deletion), delete the whole gene of nlp-11 on N2 by CRISPR/Cas9 system. |
| PHX646 | ahcy-1(syb646) | syb646 is a GFP knock-in inserted after the initiation codon of endogenous ahcy-1 gene on N2 by CRISPR/Cas9 system. |
| PHX649 | fat-6(syb649)IV | syb649 is a GFP knock-in inserted before the termination codon of endogenous fat-6 gene on N2 by CRISPR/Cas9 system. |
| PHX679 | vglu-3(syb679) | syb679 is a 3xGAS-GFP knock-in inserted before the termination codon of endogenous vglu-3 gene on N2 by CRISPR/Cas9 system. |
| PHX700 | ilys-4(syb700) | syb700 is null allele (1270bp deletion), delete the whole gene of ilys-4 on N2 by CRISPR/Cas9 system. |
| PHX731 | vha-13(syb731) | syb731 is a wrmScarlet knock-in inserted before the initiation codon of endogenous vha-13 gene on N2 by CRISPR/Cas9 system. |
| PHX762 | nlp-8(syb762)I;nlp-32(syb431)cnc-6(syb393)III;Y43C5A.3 (syb761)IV | syb762 is null allele with the whole gene of nlp-8 deleted.
syb431 is null allele with the whole gene of nlp-32 deleted.
syb393 is null allele with the whole gene of cnc-6 deleted.
syb761 is null allele with the whole gene of Y43C5A.3 deleted.
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| PHX764 | sqst-1(syb764) | syb764 is deleted allele with 2468bp sequence of C53A5.6 deleted and extra 36bp sequence inserted on N2 background strain. |
| PHX784 | ahcy-1(syb784 syb646) | syb646 is a GFP knock-in inserted after the initiation codon of endogenous ahcy-1 gene on N2 by CRISPR/Cas9 system.
syb784 is the 169th aa of the T07A9.6.1(daf-18) protein mutate from C to S on syb646 strain by CRISPR/Cas9 system. |
| PHX893 | F13E9.1 (syb767) | syb767 is deletion allele (873bp deletion), delete part of the gene of F13E9.1(introduces a new termination codon to the gene) on N2 by CRISPR/Cas9 system. F13E9.1 (syb767) strain is outcrossed 4 times.
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| PHX945 | F13E9.1(syb767);sybIs62 | syb767 is deletion allele (873bp deletion), delete part of the gene of F13E9.1(introduces a new termination codon to the gene) on N2 by CRISPR/Cas9 system. F13E9.1 (syb767) is outcrossed 4 times.
sybIs62 [NISH-1::EGFP::3xFLAG + unc-119(+) + myo- 2p::mCherry]. |
| PHX1027 | mul-1(syb1027) | syb1027 is null allele (1650bp deletion), delete the whole gene of mul-1 on N2 by CRISPR/Cas9 system.
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| PHX1048 | hdl-1(syb1048) | syb1048 is a GFP knock-in inserted before the termination codon of endogenous hdl-1 gene on N2 by CRISPR/Cas9 system.
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| PHX1049 | vha-13(syb1049) | syb1049 is a GFP-GRRIPGLIN knock-in inserted before the initiation codon of endogenous vha-13 gene on N2 by CRISPR/Cas9 system.
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| PHX1165 | C53A5.6(syb1165)/nT1 | syb1165 is null allele (2143bp deletion) with the whole gene of C53A5.6 deleted on N2 background strain.The allele is balanced by nT1[qIs51]. Heterozygotes are wild-type worms with pharynx GFP and segregate wild type worms with pharynx GFP. Pick wild-type GFP+ to maintain the strain.
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| PHX1260 | aspm-1(syb1260)klp-15(ok1958)klp-16(or1952)/hT2[bli-4(e937) let-?(q782) qIs48] | syb1260 is a GFP knock-in inserted before the initiation codon of endogenous aspm-1 gene on klp-15(ok1958) klp-16(or1952) background strain by CRISPR/Cas9 system.The allele is balanced by hT2[bli-4(e937) let-?(q782) qIs48]. Heterozygotes are wild-type worms with pharynx GFP and segregate wild type worms with pharynx GFP. Pick wild-type GFP+ to maintain the strain.
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| PHX1364 | hsp-12.6(syb1364) | syb1364 is a GSGSG-mKate2 knock-in inserted before the termination codon of endogenous hsp-12.6 gene on N2 by CRISPR/Cas9 system.
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| PHX1433 | flp-11(syb1433) | syb1433 is a SL2-egl-23cDNA(A383V)-GSGSGSGSG-mKate2 knock-in inserted after the termination codon of endogenous flp-11 gene on N2 by CRISPR/Cas9 system.
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| PHX1445 | aptf-1(gk794)II;flp-11(syb1445)X | syb1445 is a SL2(gpd-2)-unc-58(L428F)-linker(GSGSGSGSG)-mKate2(two introns) knock-in inserted after the termination codon of endogenous flp-11 gene on HBR227 aptf-1(gk794)II strain by CRISPR/Cas9 system..
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| PHX1446 | nlp-8(syb762) I; nlp-32(syb431) cnc-6(syb393) III; Y43C5A.3 (syb761) IV; nlp-25(syb579) cnc-10(syb937) cnc-7(syb558) sybDf1 sybDf2 V | PHX1446 carries knockouts of 19 members of the nlp and cnc peptide families.
sybDf1 is a deletion of a gene cassette including nlp-34, nlp-31, nlp-30, nlp-29, nlp-28, and nlp-27.
sybDf2 is a deletion of a gene cassette including cnc-11, cnc-1, cnc-5, cnc-4, cnc-3, and cnc-2.
syb762 is null allele with the whole gene of nlp-8 deleted.
syb431 is null allele with the whole gene of nlp-32 deleted.
syb393 is null allele with the whole gene of cnc-6 deleted.
syb761 is null allele with the whole gene of Y43C5A.3 deleted.
syb579 is null allele with the whole gene of nlp-25 deleted.
syb937 is null allele with the whole gene of cnc-10 deleted.
syb558 is null allele with the whole gene of cnc-7 deleted.
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| PHX1464 | flp-11(syb1464) | syb1464 is a SL2-egl-23cDNA(L229N)-GSGSGSGSG-mKate2 knock-in inserted after the termination codon of endogenous flp-11 gene on N2 by CRISPR/Cas9 system.
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| PHX1516 | daf-18(syb1516) | Mutate 169th aa of the T07A9.6.1(daf-18) protein to generate a C to S mutation on N2.
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| PHX1551 | ceh-51(syb1551) | syb1551 is a GFP knock-in inserted before the termination codon of endogenous ceh-51 gene on N2 by CRISPR/Cas9 system.
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| PHX1587 | tab-1(syb1587) | syb1587 is a GFP knock-in inserted before the termination codon of endogenous tab-1 gene on N2 by CRISPR/Cas9 system.
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| PHX1592 | ceh-5(syb1592) | syb1592 is a GFP knock-in inserted before the termination codon of endogenous ceh-5 gene on N2 by CRISPR/Cas9 system.
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| PHX1608 | ceh-24(syb1608) | syb1608 is a GFP knock-in inserted before the termination codon of endogenous ceh-24 gene on N2 by CRISPR/Cas9 system..
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| PHX1610 | ceh-92(syb1610) | syb1610 is a GFP knock-in inserted before the termination codon of endogenous ceh-92 gene on N2 by CRISPR/Cas9 system.
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| PHX1615 | daf-18(syb1615) | Mutate 137th aa of the T07A9.6.1(daf-18) protein to generate a D to A mutation on N2.
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| PHX1618 | daf-18(syb1618) | Mutate 174th aa of the T07A9.6.1(daf-18) protein to generate a G to E mutation on N2.
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| PHX1656 | ceh-8(syb1656) | syb1656 is a GFP knock-in inserted before the termination codon of endogenous ceh-8 gene on N2 by CRISPR/Cas9 system.
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| PHX1658 | unc-4(syb1658) | syb1658 is a GFP knock-in inserted before the termination codon of endogenous unc-4 gene on N2 by CRISPR/Cas9 system.
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| PHX1679 | ttx-1(syb1679) | syb1679 is a GFP knock-in inserted before the termination codon of endogenous ttx-1 gene on N2 by CRISPR/Cas9 system.
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| PHX1772 | pod-2(syb1772) | syb1772 is a GGGGG-10xHis tag knock-in inserted before the termination codon of endogenous pod-2 gene on N2 by CRISPR/Cas9 system..
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| PHX1805 | ser-1(syb1805) | syb1805 is a T2aPeptide-mNeonGreen knock-in inserted before the termination codon of endogenous ser-1 gene on N2 by CRISPR/Cas9 system.
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| PHX1812 | C03C11.8(syb1812) cfi-1(kas16[cfi-1::mNG::AID]) I | syb1812 is deletion allele (769bp deletion), delete part of the C03C11.8 gene on KRA345 strain by CRISPR/Cas9 system.
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| PHX1841 | egl-9(syb1841) | syb1841 is a wrmscarlet-AID-2xHA knock-in inserted before the termination codon of endogenous egl-9 gene on N2 by CRISPR/Cas9 system.
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| PHX1849 | ceh-23(syb1849) | syb1849 is a GFP knock-in inserted before the termination codon of endogenous ceh-23 gene on N2 by CRISPR/Cas9 system.
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| PHX1856 | C03C11.8(syb1856) cfi-1(kas16[cfi-1::mNG::AID]) | syb1856 is a gene replacement editing with C03C11.8 gene deletion (386bp) and COE1 to COE8 insertion (Eight COE motifs in A4e) on KRA345 strain by CRISPR/Cas9 system.
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| PHX1866 | ser-4(syb1866) | syb1866 is a T2A-mNeonGreen knock-in inserted before the termination codon of endogenous ser-4 gene on N2 by CRISPR/Cas9 system.
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| PHX1867 | ser-5(syb1867) | syb1867 is a T2A-mNeonGreen knock-in inserted before the termination codon of endogenous ser-5 gene on N2 by CRISPR/Cas9 system.
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| PHX1884 | ceh-75(syb1884) | syb1884 is a GFP knock-in inserted before the termination codon of endogenous ceh-75 gene on N2 by CRISPR/Cas9 system.
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| PHX1941 | ser-7(syb1941) | syb1941 is a T2A-mNeonGreen knock-in inserted before the termination codon of endogenous ser-7 gene on N2 by CRISPR/Cas9 system.
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| PHX1995 | T12E12.3(syb1995) | syb1995 is a 3xflag-GFP knock-in inserted before the termination codon of endogenous T12E12.3 gene on N2 by CRISPR/Cas9 system.
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