| Strain Name | PHX1445 |
| Genotype | aptf-1(gk794)II;flp-11(syb1445)X |
| Description | syb1445 is a SL2(gpd-2)-unc-58(L428F)-linker(GSGSGSGSG)-mKate2(two introns) knock-in inserted after the termination codon of endogenous flp-11 gene on HBR227 aptf-1(gk794)II strain by CRISPR/Cas9 system..
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| Wild type Sequence | >Wild type:
GAACAACTCCTGAGGCGAATTCATCATTAA*aaatcatatgtttttctctctcacactctc |
| Mutant Sequence |
>syb1445:
GAACAACTCCTGCGACGAATTCATCATTAAgctgtctcatcctactttcacctagttaactgcttgtcttaaaatctatgcttctctttagtatctaaaattttcctagaagcttacaagtatataaatggtctcttctcaataaaggttgtatatttattcatcttattgaatctgccatttcctcgtttttgcgagtttatataccttccaattttctttctattgtattttcaacttctaattttaattcagggaaactgcttcaacgcatcATGGCTCCACTGACTGTGAAAAGCTCACCTCCAAAAAAGGCAAAAGGAATATCAAAATTTCGGAGAAAAAAGAAGCAGCCACCACCAGACTCAACCGTATTCGTCGCATGGGCACTCCGAAGTGTCCGAGAGTCTCTGATCCAAGTTGATCCATTGGCCGCTGCACTTGCACATCAGGCTCGAAAGACAAATAGTGTGCCAGCTGTCTCGAGAACTCCACTGCTTCTACAGTTCACTCCTTTCGGACCACCTCTCAGTGCGTATCATGTGACAGCTCGGTGGGAAGGTGCAAATATCAATTCACAATCAGCATTGCTCGATGCAGATGATGGAGCTACAGTTATCACAGATACCATCAAAGATGACCAAGATGATAAAGAACCAAAAAGCTGCCCGCAACAGACTGTCAAATACATCAAAATACTTACACCTCACGTGATCTTGGTGTCAGTGTTAATTGGATATTTATGCTTGGGAGCTTGGATACTCATGTTACTGGAAACAAGGACGGAACTTCTTGCCAGATCCAAAAAACTTGTCAGGTTAACAAATTTGATGTCAAACTTCACTGCCGAAAGTTGGAAGATGCTCAATAATGCTCAACACGGGGTTAGTAATATGGATGAAGGTGAATGGGCTGCAACATTTCGAGAATGGATGGTACGAGTATCAGAAACAGTGGACGATAGGAGACCTATACGACGTGAATTAAACCGGCCTGATGACTTATCAAATATGCATAATAAATGGACATTTCCAACTGCAATATTATATGTTCTCACTGTGTTAACTACTTGCGGTTATGGAGAAGTATCTGTCGACACAGACGTCGGAAAGGTTTTCTCAGTAGCATTCGCGCTTGTTGGTATACCACTTATGTTCATAACAGCTGCCGATATTGGTAAATTTTTATCTGAAACATTACTCCAGTTTGTGAGCTTTTGGAATCGAAGTGTCCGAAAAGTGAAGCAATGGATGAGTCGTATTCGTCACGGCAGGAGAAAGTCATTACAATCAACGGGTGGTCCCAACGATACTCTCGATATTCTTGGTGTCGACGGAACTGAAGAGAAACTTTGGTTCCCAATAGGTGCATATGTATCATGTATTTGCATATATTGCTCAATTGGGTCTGCCATGTTTATCACATGGGAAAGAACTTGGTCTTTCATTCATGCGTTTCATTTTGGTTTCAATTTGATTGTAACAGTCGGACTCGGAGATATCGTTGTGACTGATTACATATTTTTATCACTTATCGTTGCATTTGTGATAGTTGGTTTTTCCGTAGTGACCATGTGCGTGGATCTTGCGTCCACACATCTCAAGGCGTACTTCACCAGAATTCACTACTTTGGTCGAGCAAAACGATTCTTAGGAATGAGTGAGGAACTCAAAGAAATCGTTGCTTTACTGGGGGCGATGCGACGGAAAAAAGGCGGTAAAGTTACATGGAATGATGTGCGAGACTTCCTGGATAACGAACTCCGCGATCGACCTTTTGAACCTCATGAGCTTCTGATGAAGCTCAGATTTATTGACGAAACATCTTCTGGAATGTCTACAATCCGTCACAATTCCTTCCAGTCAGATTTTTTCCGAGAATCAGAGTACATCCGAAGAGTGGCTGCGCTGAGGCCAGAACAGCCAGCATATTTGGGATCCGGATCCGGATCCGGATCCGGAATGTCCGAGCTCATCAAGGAGAACATGCACATGAAGCTCTACATGGAGGGAACCGTCAACAACCACCACTTCAAGTGCACCTCCGAGGGAGAGGGAAAGCCATACGAGGGAACCCAAACCATGCGTATCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGCCGTCGAGGGAGGACCACTCCCATTCGCCTTCGACATCCTCGCCACCTCCTTCATGTACGGATCCAAGACCTTCATCAACCACACCCAAGGAATCCCAGACTTCTTCAAGCAATCCTTCCCAGAGGGATTCACCTGGGAGCGTGTCACCACCTACGAGGACGGAGGAGTCCTCACCGCCACCCAAGACACCTCCCTCCAAGACGGATGCCTCATCTACAACGTCAAGATCCGTGGAGTCAACTTCCCATCCAACGGACCAGTCATGCAAAAGAAGACCCTCGGATGGGAGGCCTCCACCGAGACCCTCTACCCAGCCGACGGAGGACTCGAGGGACGTGCCGACATGGCCCTCAAGCTCGTCGGAGGAGGACACCTCATCTGCAACCTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCACCTACCGTTCCAAGAAGCCAGCCAAGAACCTCAAGATGCCAGGAGTCTACTACGTCGACCGTCGTCTCGAGCGTATCAAGGAGGCCGACAAGGAGACCTACGTCGAGCAACACGAGGTCGCCGTCGCCCGTTACTGCGACCTCCCATCCAAGCTCGGACACCGTTAAaaatcatatgtttttctctctcacactctc |
| Phenotype |