| Strain Name | PHX1165 |
| Genotype | C53A5.6(syb1165)/nT1 |
| Description | syb1165 is null allele (2143bp deletion) with the whole gene of C53A5.6 deleted on N2 background strain.The allele is balanced by nT1[qIs51]. Heterozygotes are wild-type worms with pharynx GFP and segregate wild type worms with pharynx GFP. Pick wild-type GFP+ to maintain the strain.
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| Wild type Sequence | >Wild type:
tttcacacacattgaacctcttttcagacaagacgggtcaacagttggttcaaacacaataattttcaagaaATGTCAATTGTGAATACAACAGAATGCATAATTTGTTATAATGAATATGgtgagcacattaatgaatttcgaaaaaaacttctaaatttcagACACCGAACTCCGGAAACCTTGCATAGGCACATGCGGCCACTCCATATGTGAAAGTTGCAAACATCAAATGGTCTCATCAAAATGTCCACAGTGCAATCGAGAAGAAGCCTTCGCAATTACTACAATCAATTATCAAGTTTTAGAATTAATAAAGCACTTTAAAAGTATGCAAAGTGGGGGAGTAGCGCCACAGGCACAATCCACAAATgtaagtataatttcctttttctataaaaacccagaaaaagtttttgcagAGCTTAGACGAAGGAACATGCAGTGAGTGCACCCTACATTCCAAGAAATTACGAATTTGTAAAACGTGCGCCGTCGGAAAAGGAGTTCTGAAGCAAGCCGAAGGCAAAAAAGAATTAGTATTAGTTGTTGGAGATGATGGAGTCGATGAAGCAATGACACGAGCTAAGAAGATGGCGATTTGCGGTGATTGTGCTTTGGATGGGGCCCAACACGAAGGACATCAGACAATGCAGCTGGCTCTTTTGAAAAATAGTATCGAAGATCAAGCACCGACTAATCTCGGAAATAAAATCAGTAAAATCAGTGAGCTTTCGAAGCAGGTGTGCGATGATATCTCTGACAAATTTGCGGCGACAATTAAATCACTTCAAAATTACTTCGAAAAGCATGAAAAGTTGTCTCCGCCCGGAAGAAAGACTCACTTCAACAACATCGAAGCTGAAATGGAGAAAATAAGTGATACGATAGCTCTTGTCAAAGATGCTGGAACACGATTGGATTTTTTGAATAACGAAATCGAAGAGCACGATCGAATCGTTTTTCAAACTAAACAACCTGTTACTAAGGCAAATTTCGTTTTCGCTAGTCAGGATCCCGTATTGGTTGCAGgtaaaagttatatttatgttgtgcattcttgaacgaaaaaagctcatcgcgacgctcgcgtgtcgttcgttgttatcatgcttttgcattttatacagactcctcccgcgattccagtctaaaaaatctctcaaaattattctttttttccagATCGAAAGCAGAGTGCCCGTGAACCACTTTTGATTGGAGTCTACGATCCTAAAAACAGAGGATGGACGCCAGTTGGGCGAATGCCGAACCCAAAATCAAACTACGCTGTTGCCTGCAGCAAATCTCAAATTTATATAGTCGGAGGAATGAATAACGGATCATGGTTGAACAACGTTGAAATGTATGATCGGGAGAAAAACTTGCGAAGAGATgtgggttattctttcagcttttaaaaaattctctagaattgtaaacttttaaaacaaataatttttattgatttcaacaaaaatgtcaaagttattacagTGTAATAAAATGAAGCGTGGAAGAACACGAACGTCGGCGGGAATCCATAACAACAAAATGTACGTGGCCGGTGGATATGATAGCACTTATATGAATTCTGTGGAAGTTTATGATACTGATCAAGGAGAGTGGAAGGATGGACCATCGTTGCAAAGGCCTCGTGCCGATGCAGCGGTTGTTCCATGCAATGGAGAGGTTTATGgtatgatttcgattaattagttgattaaaaaaggtttaatttcagTGTTGGGAGGTTTCAATGGAAAAGAATATGAAGAGAAGATTGAGAAGCTGTCAGAACCTGGTCAAAAGTTCGAAATTGTGGGCGACATGCAAGGAAGTCGCGCCGGATTTGGATCGTGTGCCTTCAGAGGAAGAATCTACATTGCTGGTGGATGGAGTAACTCGAGTAATACGTTGAGATCAGTTCGAAGCTATGATCCATTGACGAAATCATGGAGAGATGAGCCATCGTTGAGCAAAGATCGAAAATATTTCACTCTTCACTCGACGAACGAAGCTATTTATGCTATTCGCGGATGTGCTGACAATTGGAGCTTAATCAATGAAGTCGAGCGATTTGATCCAGAAAAACGTGCATGGGAGATTATTAGTTGCTCCGGACCAATGGTTGGAGCCGGTTCATCTGCATCGAATGAGCAAGCTATTACATAAaatcgtgtatcttattttcataaaaacagctgtt |
| Mutant Sequence |
>syb1165(-2143bp):
tttcacacacattgaacctcttttcagacaag-aatcgtgtatcttattttcataaaaacagctgtt |
| Phenotype | Balanced heterozygotes are wild-type GFP+ . |