| Strain Name | PHX3364 |
| Genotype | eif-2D(syb3364) |
| Description | syb3364 is a GFPnovo2-3xFlag knock-in inserted before the termination codon of endogenous eif-2D gene on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type:GTCGAAGGTCCTCAAGTACTCGTTCAAGGAAATCAAATTCATTTTATCGCTGATCTTCTGATAAAATCGTATGGAATCGACAAGAAATTTATGAAAGGAACCGAGTTGGCGGTGAAAAAGAAGAAA*TAAgaaaatttggagaaatttgcaaattca |
| Mutant Sequence |
>syb3364:GTAGAAGGTCCTCAAGTTCTCGTTCAAGGAAATCAAATTCATTTTATCGCTGATCTTCTGATAAAATCGTATGGAATCGACAAGAAATTTATGAAAGGAACCGAGTTGGCCGTGAAAAAGAAGAAAATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTTCAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGCTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGACCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTGTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGATTATAAAGACGATGACGATAAGCGTGACTACAAGGACGACGACGACAAGCGTGATTACAAGGATGACGATGACAAGAGATAAgaaaatttggagaaatttgcaaattca |
| Phenotype |