| Strain Name | PHX3426 |
| Genotype | ceh-27(syb2714 syb3426) |
| Description | syb2714 is a LoxP knock-in inserted 913bp downstream of initiation codon of endogenous ceh-27 gene on N2 by CRISPR/Cas9 system..
syb3426 is a GFP(LoxP) knock-in inserted before the termination codon of endogenous ceh-27 gene on syb2714 strain by CRISPR/Cas9 system..
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| Wild type Sequence | >Wild type1:
Cgtcctacagtacctccccttggttgccac*aacattggaaatgaacaagtttgccaactc
>Wild type2:
GCAAACTATCCTCCGTCTACATACAATTTATCGACATCAGCATTTCATAACCTT*TAGctgacattcaactatgctcaatttttt |
| Mutant Sequence |
>syb2714:
cgtcctacagtacctccccttggttgccacATAACTTCGTATAGCATACATTATACGAAGTTATaacattggaaatgaacaagtttgccaactc
>syb3426:
GCTAACTATCCTCCGTCTACATACAATTTATCGACATCAGCATTTCATAACCTTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaacATAACTTCGTATAGCATACATTATACGAAGTTATtaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGctgacattcaactatgctcaatttttt |
| Phenotype |