| Strain Name | PHX3432 |
| Genotype | eif-2D(syb3432) |
| Description | syb3432 is a gene replacement editing with part of eif-2D gene deletion(332bp) and 3xFlag insertion on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type:AGAATGACTGCAACATGGCACATAAGATGGCCTGACGGCAGAGAAATAGTCAGAAAAGTATCTCCACCGAGAGTTGAATTCAAGATCGAAAACCGTGCTGGAAACAAAAAAGTTACTCTTTTAAATGGATTAGCGATGTTTGGAATTGACATTCGAACGATTTGCCATCAAATTCAGgtagttggtggctaatccttaaatactaatttctgaattttcagACTGGTGTGGCTACTAGTGTTACAAGTCAATGGGAAGTTCCTGGCGTCGAAGGTCCTCAAGTACTCGTTCAAGGAAATCAAATTCATTTTATCGCTGATCTTCTGATAAAATCGTATGGAATCGACAAGAAATTTATGAAAGGAACCGAGTTGGCGGTGAAAAAGAAGAAATAAgaaaatttggagaaatttgcaaattca |
| Mutant Sequence |
>syb3432:AGAATGACTGCAACATGGCACATAAGATGGCCTGACGGCAGAGAAATAGTCAGAAAAGTCGACTATAAAGACGATGACGATAAGCGTGACTACAAGGACGACGACGACAAGCGTGATTACAAGGATGACGATGACAAGAGATAAgaaaatttggagaaatttgcaaattca |
| Phenotype |