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Strain Name
PHX3596
Genotype
pah-1(syb3596) tph-1(mg280)
Description
syb3596 is deletion allele (1450bp deletion), delete part of the gene of pah-1 on tph-1(mg280) strain by CRISPR/Cas9 system.
Wild type Sequence
>Background:cctctgaaaaccaaatcttgttctctgaaaATGCCACCAGCTGGACAAGATGATCTTGACTTCTTGgtgagaccttccttgtttgaactgatcaagaaaaacttctgtttgagaattttgtgtaaaattatttccaattactaaagtccaacttatttcagAAGTACGCCATGGAATCGTACGTTGCTGACGTCAACGCCGACATTGGCAAGACTACTATCGTATTCACTCTTCGTGAAAAGGCAGGAGCTCTCGCTGAAACATTGAAGCTGTTCCAGgttagtcatgaaaaaaaccagactagaaatgtcagaggatcatgagacttcagaggatgactattgcgtcagcgacacttttttttttaaatcttatgattctcacgtctactcttgatcaggcggtttaatattttatgcatcataaagcgttgaataatatgatcttcaattatgtttagacggttgagtagacatagaaaaaacatttatatcaaattaaaatttgaaaatttgcaccaaactgtttctaaatatgataagagataaactacgtagttgcattttttaatccggttcgcgatttttgattatcaaagatgtcgaaaatcgtgtcacgagaaaaagagatcatatcaattgtctcaaattcctaccaattacatgcttcattacacattcttgcagGCACATGATGTGAATCTGTCTCACATTGAATCAAGACCATCAAAGACTCATGAAGGATGCTATGAGGTGCTCGTTGAATTTGCTGAAGCTGAAGACCATCGTAAGATTGAAGGAGTTATTGAGCATTTCCAACAAAAAGCTGAAAAGAAGGTTCTTGTTCAAGACTGGAACACCAAAAACAAACAAAACAAGGgtacgctataacaataatgaaactcacagtattaaattcaagagctttgtagATTCTGTTCCATGGTTCCCACAGAAGATCAACGACATCGATCAATTTGCCAACAGAATTCTTTCTTATGGAGCTGAGCTTGATGCCGATCACCCTGGATTTAAGGACATGACCTACCGCGAGCGCAGAAAGTTTTTCGCCGATATTGCATTCAACTTCAAACAgtaagtcatttcatcaacaaaagtagcaatatgtgccaataaatatgtaacattgaaggcgtactgaataaaatccaaaaaatccgaattgcaacctgatcgtactttccagCGGAGACAAGATCCCTACTATCACCTACACTGATGAAGAAATTGCCACGTGGCGTACAGTCTACAACGAGCTGACAGTTATGTACCCGAAAAACGCTTGCCAAGAGTTCAACTACATATTCCCACTCCTCCAGCAGAATTGTGGTTTTGGACCTGACCGCATTCCACAATTGCAGGATGTTTCAGATTTTTTGAAGGgtaaacaaatcattatcatctgattgtttttgtatttgtttcgtgttcagATTGTACCGGGTACACGATTCGACCAGTCGCTGGTCTTCTTTCTTCTCGTGATTTCT
Mutant Sequence
>syb3596:cctctgaaaaccaaatcttgttctctgaaa-TCGCTGGTCTTCTTTCTTCTCGTGATTTCT
Phenotype