| Strain Name | PHX3678 |
| Genotype | pah-1(syb3678) tph-1(mg280) II |
| Description | syb3678 is a GFP-CPGDRWSSTGGGRSRTS-H2B-T2A knock-in inserted after the initiation codon of endogenous pah-1 gene on tph-1(mg280)strain by CRISPR/Cas9 system. |
| Wild type Sequence | >Background:ctgaaaaccaaatcttgttctctgaaaATG*CCACCAGCTGGACAAGATGATCTTGACTTC |
| Mutant Sequence |
>syb3678:ctgaaaaccaaatcttgttctctgaaaATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATGCCCGGGGGATCGGTGGAGCTCCACCGGTGGCGGCCGCTCTAGAACTAGTCCACCAAAGCCATCTGCCAAGGGAGCCAAGAAGGCCGCCAAGACCGTCGTTGCCAAGCCAAAGGACGGAAAGAAGAGACGTCATGCCCGCAAGGAATCGTACTCCGTCTACATCTACCGTGTTCTCAAGCAAGTTCACCCAGACACCGGAGTCTCCTCCAAGGCCATGTCTATCATGAACTCCTTCGTCAACGATGTATTCGAACGCATCGCTTCGGAAGCTTCCCGTCTTGCTCATTACAACAAACGCTCAACGATCTCATCCCGCGAAATTCAAACCGCTGTCCGTTTGATTCTCCCAGGAGAACTTGCCAAGCACGCCGTGTCTGAGGGAACCAAGGCCGTCACCAAGTACACTTCCAGCAAGGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGCCCACCACCTGCGGGACAAGATGATCTTGACTTC |
| Phenotype |