| Strain Name | PHX2541 |
| Genotype | twk-16(syb2541) |
| Description | syb2541 is a wrmScarlet-degron knock-in inserted after the initiation codon of endogenous twk-16 gene on N2 by CRISPR/Cas9 system..
|
| Wild type Sequence | >Wild type tttgcaatccatcttccagataccacaATG*ATGTTTATTCTGCAACAACTACTTCGGTTT |
| Mutant Sequence |
>syb2541:
tttgcaatccatcttccagataccacaATGGTCAGCAAGGGAGAGGCAGTTATCAAGGAGTTCATGCGTTTCAAGGTCCACATGGAGGGATCCATGAACGGACACGAGTTCGAGATCGAGGGAGAGGGAGAGGGACGTCCATACGAGGGAACCCAAACCGCCAAGCTCAAGGTCACCAAGGGAGGACCACTCCCATTCTCCTGGGACATCCTCTCCCCACAATTCATGTACGGATCCCGTGCCTTCACCAAGCACCCAGCCGACATCCCAGACTACTACAAGCAATCCTTCCCAGAGGGATTCAAGTGGGAGCGTGTCATGAACTTCGAGGACGGAGGAGCCGTCACCGTCACCCAAGACACCTCCCTCGAGGACGGAACCCTCATCTACAAGGTCAAGCTCCGTGGAACCAACTTCCCACCAGACGGACCAGTCATGCAAAAGAAGACCATGGGATGGGAGGCCTCCACCGAGCGTCTCTACCCAGAGGACGGAGTCCTCAAGGGAGACATCAAGATGGCCCTCCGTCTCAAGGACGGAGGACGTTACCTCGCCGACTTCAAGACCACCTACAAGGCCAAGAAGCCAGTCCAAATGCCAGGAGCCTACAACGTCGACCGTAAGCTCGACATCACCTCCCACAACGAGGACTACACCGTCGTCGAGCAATACGAGCGTTCCGAGGGACGTCACTCCACCGGAGGAATGGACGAGCTCTACAAGatgcctaaagatccagccaaacctccggccaaggcacaagttgtgggatggccaccggtgagatcataccggaagaacgtgatggtttcctgccaaaaatcaagcggtggcccggaggcggcggcgttcgtgaagATGTTTATTCTGCAACAACTACTaCGGTTT |
| Phenotype |