| Strain Name | PHX509 |
| Genotype | nhr-67(syb509) |
| Description | syb509 is a GASGASGAS-GFP-3xFlag knock-in inserted before the termination codon of endogenous nhr-67 gene on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type
CGTCATTCGATTCGATCAATAACTGAATTATTATCAATTCAAGAAGAAGAGAGTGTTAATGTTGAAGAGGTT*TAGatagtaaattcatgtttcatatacagt |
| Mutant Sequence |
>syb509 AGGCATTCGATTCGATCAATAACTGAATTATTATCAATTCAAGAAGAAGAGAGTGTTAATGTTGAAGAAGTTGGAGCATCGGGAGCCTCAGGAGCATCGATGAGTAAAGGAGAAGAATTGTTCACTGGAGTTGTCCCAATCCTCGTCGAGCTCGACGGAGACGTCAACGGACACAAGTTCTCCGTCTCCGGAGAGGGAGAGGGAGACGCCACCTACGGAAAGCTCACCCTCAAGTTCATCTGCACCACCGGAAAGCTCCCAGTCCCATGGCCAACCCTCGTCACCACCTTCTGCTACGGAGTCCAATGCTTCTCCCGTTACCCAGACCACATGAAGCGTCACGACTTCTTCAAGTCCGCCATGCCAGAGGGATACGTCCAAGAGCGTACCATCTTCTTCAAGgtaagtttaaacatatatatactaactactgattatttaaattttcagGACGACGGAAACTACAAGACCCGTGCCGAGGTCAAGTTCGAGGGAGACACCCTCGTCAACCGTATCGAGCTCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAATCGACTTCAAGGAGGACGGAAACATCCTCGGACACAAGCTCGAGTACAACTACAACTCCCACAACGTCTACATCATGGCCGACAAGCAAAAGAACGGAATCAAGGTCAACTTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagATCCGTCACAACATCGAGGACGGATCCGTCCAACTCGCCGACCACTACCAACAAAACACCCCAATCGGAGACGGACCAGTCCTCCTCCCAGACAACCACTACCTCTCCACCCAATCCGCCCTCTCCAAGGACCCAAACGAGAAGCGTGACCACATGGTCCTCCTCGAGTTCGTCACCGCCGCCGGAATCACCCACGGAATGGACGAGCTCTACAAGGAGAATCTGTACTTTCAATCCGGAGCCGGATCTGATTATAAAGACGATGACGATAAGCGTGACTACAAGGACGACGACGACAAGCGTGATTACAAGGATGACGATGACAAGAGATAGatagtaaattcatgtttcatatacagt |
| Phenotype |