| Strain Name | PHX5321 |
| Genotype | bli-4(syb5321) |
| Description | syb5321 is a Super folder GFP knock-in inserted before the 151st aa of endogenous bli-4 gene on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type
ACTCGTAGAGCTCAACGACAGCAGCCACAG*TCTCCACGAGAAATTCCATCACTTCCATTT |
| Mutant Sequence |
>syb5321 ACTCGTAGAGCTCAACGACAGCAGCCACAGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCcGTGGAGAGGGTGAAGGTGATGCAACAaACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTCTTACATATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGACATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATCATTCAAAGATGACGGGACTTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTTTAACTCACACAATGTATACATCACAGCAGACAAACAAAAGAATGGAATCAAAGCTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACGTTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGTCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGACGAACTATACAAATCTCCAAGGGAAATTCCATCACTTCCATTT |
| Phenotype |