| Strain Name | PHX5400 |
| Genotype | T24B1.1(syb5400) |
| Description | syb5400 is a wrmScarlet knock-in inserted before the termination codon of endogenous T24B1.1 gene on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type
ATTGTTCTCACGTATACCCCAGAGATTCAT*TGAtttaatcacggttgtgttccttattat |
| Mutant Sequence |
>syb5400 ATTGTTCTCACGTATACTCCAGAGATTCATATGGTCAGCAAGGGAGAGGCAGTTATCAAGGAGTTCATGCGTTTCAAGGTCCACATGGAGGGATCCATGAACGGACACGAGTTCGAGATCGAGGGAGAGGGAGAGGGACGTCCATACGAGGGAACCCAAACCGCCAAGCTCAAGGTCACCAAGGGAGGACCACTCCCATTCTCCTGGGACATCCTCTCCCCACAATTCATGTACGGATCCCGTGCCTTCACCAAGCACCCAGCCGACATCCCAGACTACTACAAGCAATCCTTCCCAGAGGGATTCAAGTGGGAGCGTGTCATGAACTTCGAGGACGGAGGAGCCGTCACCGTCACCCAAGACACCTCCCTCGAGGACGGAACCCTCATCTACAAGGTCAAGCTCCGTGGAACCAACTTCCCACCAGACGGACCAGTCATGCAAAAGAAGACCATGGGATGGGAGGCCTCCACCGAGCGTCTCTACCCAGAGGACGGAGTCCTCAAGGGAGACATCAAGATGGCCCTCCGTCTCAAGGATGGAGGACGTTACCTCGCCGACTTCAAGACCACCTACAAGGCCAAGAAGCCAGTCCAAATGCCAGGAGCCTACAACGTCGACCGTAAGCTCGACATCACCTCCCACAACGAGGACTACACCGTCGTCGAGCAATACGAGCGTTCCGAGGGACGTCACTCCACCGGAGGAATGGACGAGCTCTACAAGTAGtttaatcacggttgtgttccttattat |
| Phenotype |