Strain Name | PHX4741 |
Genotype | dat-1(syb4741) |
Description | syb4741 is a T2A::mNeonGreen knock-in inserted before the termination codon of endogenous dat-1 gene on N2 by CRISPR/Cas9 system..
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Wild type Sequence | >Wild type
ACCACTCAACCGCACTCTGACATAATGCTA*TGAacataggtgtcttccactttcattatc |
Mutant Sequence |
>syb4741:
ACCACTCAACCTCATTCTGACATAATGCTAGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGCCCAGTCTCCAAGGGAGAGGAGGACAACATGGCCTCCCTCCCAGCCACCCACGAGCTCCACATCTTCGGATCCATCAACGGAGTCGACTTCGACATGGTCGGACAAGGAACCGGAAACCCAAACGACGGATACGAGGAGCTCAACCTCAAGTCCACCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGGAGACCTCCAATTCTCCCCATGGATCCTCGTCCCACACATCGGATACGGATTCCACCAATACCTCCCATACCCAGACGGAATGTCCCCATTCCAAGCCGCCATGGTCGACGGATCCGGATACCAAGTCCACCGTACCATGCAATTCGAGGACGGAGCCTCCCTCACCGTCAACTACCGTTACACCTACGAGGGATCCCACATCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAGAGGCCCAAGTCAAGGGAACCGGATTCCCAGCCGACGGACCAGTCATGACCAACTCCCTCACCGCCGCCGACTGGTGCCGTTCCAAGAAGACCTACCCAAACGACAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCATCATCTCCACCTTCAAGTGGTCCTACACCACCGGAAACGGAAAGCGTTACCGTTCCACCGCCCGTACCACCTACACCTTCGCCAAGCCAATGGCCGCCAACTACCTCAAGAACCAACCAATGTACGTCTTCCGTAAGACCGAGCTCAAGCACTCCAAGACCGAGCTCAACTTCAAGGAGTGGCAAAAGGCCTTCACCGACGTCATGGGAATGGACGAGCTCTACAAGTGAacataggtgtcttccactttcattatc |
Phenotype |