Strain Name | PHX5759 |
Genotype | gbb-2(syb5759) |
Description | syb5759 is a SL2-GFP-H2B knock-in inserted after the termination codon of endogenous gbb-2 gene on N2 by CRISPR/Cas9 system. |
Wild type Sequence | >Wild type
GATCCGGATGAGCCGAGCACAAAGCTTTAG*gattcttcaattttttttgaattttaaaag |
Mutant Sequence |
>syb5759 GACCCTGATGAGCCGAGCACAAAGTTGTAGgctgtctcatcctactttcacctagttaactgcttgtcttaaaatctatgcttctctttagtatctaaaattttcctagaagcttacaagtatataaatggtctcttctcaataaaggttgtatatttattcatcttattgaatctgccatttcctcgtttttgcgagtttatataccttccaattttctttctattgtattttcaacttctaattttaattcagggaaactgcttcaacgcatcATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAACCACCAAAGCCATCTGCCAAGGGAGCCAAGAAGGCCGCCAAGACCGTTACGAAGCCAAAGGACGGAAAGAAGAGACGTCATGCCCGTAAGGAATCATACTCCGTCTACATCTACCGTGTCCTCAAGCAAGTTCATCCAGACACTGGAGTTTCCTCCAAAGCCATGTCTATCATGAACTCTTTTGTCAACGATGTCTTCGAGCGTATTGCTGCTGAAGCATCCCGTCTTGCTCACTACAACAAGCGTTCCACAATCTCATCCCGCGAAATTCAGACCGCTGTCCGTCTGATCCTTCCAGGAGAGCTTGCCAAGCACGCCGTGTCTGAGGGAACCAAGGCCGTTACCAAGTACACTTCCAGCAAGTAGgattcttcaattttttttgaattttaaaag |
Phenotype |