Strain Name | PHX5791 |
Genotype | pop-1(syb5791) |
Description | syb5791 is a GFP-AID-GGGGSGSGS knock-in inserted after the initiation codon of endogenous pop-1 gene on N2 by CRISPR/Cas9 system. |
Wild type Sequence | >Wild type
AatttccagatataaaaatacacaaaaATG*ATGGCCGACGAAGAGCTCGGCGATGAGGTG |
Mutant Sequence |
>syb5791 aatttccagatataaaaatacacaaaaATGAGTAAAGGAGAAGAATTGTTCACTGGAGTTGTCCCAATCCTCGTCGAGCTCGACGGAGACGTCAACGGACACAAGTTCTCCGTCTCCGGAGAGGGAGAGGGAGACGCCACCTACGGAAAGCTCACCCTCAAGTTCATCTGCACCACCGGAAAGCTCCCAGTCCCATGGCCAACCCTCGTCACCACCTTCTGCTACGGAGTCCAATGCTTCTCCCGTTACCCAGACCACATGAAGCGTCACGACTTCTTCAAGTCCGCCATGCCAGAGGGATACGTCCAAGAGCGTACCATCTTCTTCAAGGTAAGTTTAAACATATATATACTAACTACTGATTATTTAAATTTTCAGGACGACGGAAACTACAAGACCCGTGCCGAGGTCAAGTTCGAGGGAGACACCCTCGTCAACCGTATCGAGCTCAAGGTAAGTTTAAACAGTTCGGTACTAACTAACCATACATATTTAAATTTTCAGGGAATCGACTTCAAGGAGGACGGAAACATCCTCGGACACAAGCTCGAGTACAACTACAACTCCCACAACGTCTACATCATGGCCGACAAGCAAAAGAACGGAATCAAGGTCAACTTCAAGGTAAGTTTAAACATGATTTTACTAACTAACTAATCTGATTTAAATTTTCAGATCCGTCACAACATCGAGGACGGATCCGTCCAACTCGCCGACCACTACCAACAAAACACCCCAATCGGAGACGGACCAGTCCTCCTCCCAGACAACCACTACCTCTCCACCCAATCCGCCCTCTCCAAGGACCCAAACGAGAAGCGTGACCACATGGTCCTCCTCGAGTTCGTCACCGCCGCCGGAATCACCCACGGAATGGACGAGCTCTACAAGATGCCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGCCCGGAGGCGGCGGCGTTCGTGAAGGGTGGTGGCGGTTCTGGCTCAGGATCTATGGCCGACGAAGAGtTgGGCGATGAGGTG |
Phenotype |