Strain Name | PHX5804 |
Genotype | egl-3(syb5804) |
Description | syb5804 is a Flag-NLS-CRE-SL2 knock-in inserted before the initiation codon of endogenous egl-3 gene on N2 by CRISPR/Cas9 system. |
Wild type Sequence | >Wild type
Attgacaataaatatttttcaattgaaggt*ATGAAAAACACACATGTCGACCTAATATGTGTGTTCCTGTCGATTTTCATCGGC |
Mutant Sequence |
>syb5804 attgacaataaatatttttcaattgaaggtATGGACTACAAGGACCACGATGGAATGTCCCGTCGTCGTAAGGCCAACCCAACCAAGCTCTCCGAGAACGCCAAGAAGCTCGCCAAGGAGGTCGAGAACGGAGGAATGTCCAACCTCCTCACCGTCCACCAAAACCTCCCAGCCCTCCCAGTCGACGCCACCTCCGACGAGGTCCGTAAGAACCTCATGGACATGTTCCGTGACCGTCAAGCCTTCTCCGAGCACACCTGGAAGATGCTCCTCTCCGTCTGCCGTTCCTGGGCCGCCTGGTGCAAGCTCAACAACCGTAAGTGGTTCCCAGCCGAGCCAGAGGACGTCCGTGACTACCTCCTCTACCTCCAAGCCCGTGGACTCGCCGTCAAGACCATCCAACAACACCTCGGACAACTCAACATGCTCCACCGTCGTTCCGGACTCCCACGTCCATCCGACTCCAACGCCGTCTCCCTCGTCATGCGTCGTATCCGTAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGAGAACGTCGACGCCGGAGAGCGTGCCAAGCAAGCCCTCGCCTTCGAGCGTACCGACTTCGACCAAGTCCGTTCCCTCATGGAGAACTCCGACCGTTGCCAAGACATCCGTAACCTCGCCTTCCTCGGAATCGCCTACAACACCCTCCTCCGTATCGCCGAGATCGCCCGTATCCGTGTCAAGGACATCTCCCGTACCGACGGAGGACGTATGCTCATCCACATCGGACGTACCAAGACCCTCGTCTCCACCGCCGGAGTCGAGAAGGCCCTCTCCCTCGGAGTCACCAAGCTCGTCGAGCGTTGGATCTCCGTCTCCGGAGTCGCCGACGACCCAAACAACTACCTCTTCTGCCGTGTCCGTAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagAACGGAGTCGCCGCCCCATCCGCCACCTCCCAACTCTCCACCCGTGCCCTTGAGGGAATCTTCGAGGCCACCCACCGTCTCATCTACGGAGCCAAGGACGACTCCGGACAACGTTACCTCGCCTGGTCCGGACACTCCGCCCGTGTCGGAGCCGCCCGTGACATGGCCCGTGCCGGAGTCTCCATCCCAGAGATCATGCAAGCCGGAGGATGGACCAACGTCAACATCGTTATGAACTACATCCGTAACCTCGACTCCGAGACCGGAGCTATGGTCCGTCTCCTTGAGGACGGAGACTAAgctgtctcatcctactttcacctagttaactgcttgtcttaaaatctatgcttctctttagtatctaaaattttcctagaagcttacaagtatataaatggtctcttctcaataaaggttgtatatttattcatcttattgaatctgccatttcctcgtttttgcgagtttatataccttccaattttctttctattgtattttcaacttctaattttaattcagggaaactgcttcaacgcatcATGAAAAACACACATGTCGACCTCATATGTGTGTTCCTGTCGATCTTCATCGGT |
Phenotype |