| Strain Name | PHX731 |
| Genotype | vha-13(syb731) |
| Description | syb731 is a wrmScarlet knock-in inserted before the initiation codon of endogenous vha-13 gene on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >wild type
Gctttctaaattcattcattccaggaaaag*ATGGCCGCAGAATCTTCGTACGGATTCGTTTACGGAGTGTCC |
| Mutant Sequence |
>syb731 GctttctaaattcattcattccaggaaaagATGGTATCAAAGGGCGAAGCCGTGATAAAAGAGTTCATGCGATTCAAAGTCCACATGGAGGGTTCTATGAACGGACACGAGTTTGAAATCGAAGGAGAAGGTGAGGGCCGACCTTATGAAGGCACTCAAACAGCAAAGTTAAAGGTTACGAAAgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGGAGGCCCGCTCCCTTTTTCTTGGGATATACTGTCACCGCAGTTTATGTACGGCTCTCGTGCGTTCACCAAGCATCCTGCGGATATTCCTGACTACTATAAACAATCTTTCCCTGAGGGATTCAAGTGGGAGAGAGTTATGAACTTTGAGGATGGCGGCGCAGTGACGGTGACCCAAGACACATCCCTCGAAGACGGAACCCTTATTTATAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTAAAATTGCGAGGCACAAATTTTCCACCTGATGGTCCGGTCATGCAAAAAAAGACCATGGGTTGGGAGGCTTCTACTGAGCGTCTTTATCCTGAAGATGGAGTTCTGAAAGGCGATATAAAAATGGCATTACGTTTGAAAgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagGACGGCGGCCGATATCTGGCAGATTTCAAAACTACATACAAAGCAAAGAAGCCAGTACAAATGCCGGGAGCCTATAACGTAGACCGAAAATTAGATATTACATCACATAATGAGGACTACACGGTCGTCGAACAATACGAGCGATCCGAAGGCCGTCATTCCACGGGAGGTATGGATGAGTTATACAAGATGGCCGCAGAATCTTCGTACGGATTCGTGTACGGAGTGTCG |
| Phenotype |