Strain Name | PHX893 |
Genotype | F13E9.1 (syb767) |
Description | syb767 is deletion allele (873bp deletion), delete part of the gene of F13E9.1(introduces a new termination codon to the gene) on N2 by CRISPR/Cas9 system. F13E9.1 (syb767) strain is outcrossed 4 times.
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Wild type Sequence | >wild type
CCAATCGAGTTGCATGCGATTACCGAACGACTACGGTTGCCGGAGCCAACTGCTCCAGgtatgatacatttttcaatgctgtaaaggatttttaaatgctagcgactgtggggtttttgccaaaaaaactgaaaaaagttgcaatataccgaatataaatgttaaacagtacacaattaaacaattcaaatgaccgagctatttttgaatatccgcacaaattattgacctaattccgagattttttttctttggaaaaaaattataatattttaattttctagttatttctcccattcagccgttatatattaatttttcgatatcatttattttttaatgttcttctacacatctatgaagtttgaaagtttgaaaagttgaaatacggttgaaaacatataactaaaacaaaaaagacccataaaaatattttgaaatatgttatggctacatcacttgaaaattacattcatcatcttaaacgaaattattccaagttacagATGTCAACATGGTGGCAGATATTGGGAATACCATTGACTTTCTTCACAGAGTTCGTGCTCTTAAAATCAGGGGACAGAAAGGATATGTTGGAACATCGAATATCGTTTGGAATTCACTGGATATGAGTCTCTATTTTTGCAAAGGACTGAAAGCATTATGGgtatgttcaagattttgaaaaatgtactttataaatacctattctaataataaaaaccttttttaaaatcaaattggtaatcaatttcagATCGCCGACAGTGATGTATGTCGTATTAATGGAATCAAAAGTATAAGAGAGACACTTCGTCGTCTTGTCGTTCACTATTCGATGAAAAAGATCAAGGATTTGCTCTTCGATGAAGAAGATTTGGAAACTGGAATGCTTGTGGAGGAGATGGACACGTGGAAATGTCTGGAAGAAGT |
Mutant Sequence |
>syb767(-873bp+1bp)
CCAATCGAGTTGCATGCGATTACCGAACGA-TGATGGACACGTGGAAATGTCTGGAAGAAGT |
Phenotype |