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Strain Name
PHX893
Genotype
F13E9.1 (syb767)
Description
syb767 is deletion allele (873bp deletion), delete part of the gene of F13E9.1(introduces a new termination codon to the gene) on N2 by CRISPR/Cas9 system. F13E9.1 (syb767) strain is outcrossed 4 times.
Wild type Sequence
>wild type CCAATCGAGTTGCATGCGATTACCGAACGACTACGGTTGCCGGAGCCAACTGCTCCAGgtatgatacatttttcaatgctgtaaaggatttttaaatgctagcgactgtggggtttttgccaaaaaaactgaaaaaagttgcaatataccgaatataaatgttaaacagtacacaattaaacaattcaaatgaccgagctatttttgaatatccgcacaaattattgacctaattccgagattttttttctttggaaaaaaattataatattttaattttctagttatttctcccattcagccgttatatattaatttttcgatatcatttattttttaatgttcttctacacatctatgaagtttgaaagtttgaaaagttgaaatacggttgaaaacatataactaaaacaaaaaagacccataaaaatattttgaaatatgttatggctacatcacttgaaaattacattcatcatcttaaacgaaattattccaagttacagATGTCAACATGGTGGCAGATATTGGGAATACCATTGACTTTCTTCACAGAGTTCGTGCTCTTAAAATCAGGGGACAGAAAGGATATGTTGGAACATCGAATATCGTTTGGAATTCACTGGATATGAGTCTCTATTTTTGCAAAGGACTGAAAGCATTATGGgtatgttcaagattttgaaaaatgtactttataaatacctattctaataataaaaaccttttttaaaatcaaattggtaatcaatttcagATCGCCGACAGTGATGTATGTCGTATTAATGGAATCAAAAGTATAAGAGAGACACTTCGTCGTCTTGTCGTTCACTATTCGATGAAAAAGATCAAGGATTTGCTCTTCGATGAAGAAGATTTGGAAACTGGAATGCTTGTGGAGGAGATGGACACGTGGAAATGTCTGGAAGAAGT
Mutant Sequence
>syb767(-873bp+1bp) CCAATCGAGTTGCATGCGATTACCGAACGA-TGATGGACACGTGGAAATGTCTGGAAGAAGT
Phenotype