| Strain Name | PHX945 |
| Genotype | F13E9.1(syb767);sybIs62 |
| Description | syb767 is deletion allele (873bp deletion), delete part of the gene of F13E9.1(introduces a new termination codon to the gene) on N2 by CRISPR/Cas9 system. F13E9.1 (syb767) is outcrossed 4 times.
sybIs62 [NISH-1::EGFP::3xFLAG + unc-119(+) + myo- 2p::mCherry]. |
| Wild type Sequence | >wild type
CCAATCGAGTTGCATGCGATTACCGAACGACTACGGTTGCCGGAGCCAACTGCTCCAGgtatgatacatttttcaatgctgtaaaggatttttaaatgctagcgactgtggggtttttgccaaaaaaactgaaaaaagttgcaatataccgaatataaatgttaaacagtacacaattaaacaattcaaatgaccgagctatttttgaatatccgcacaaattattgacctaattccgagattttttttctttggaaaaaaattataatattttaattttctagttatttctcccattcagccgttatatattaatttttcgatatcatttattttttaatgttcttctacacatctatgaagtttgaaagtttgaaaagttgaaatacggttgaaaacatataactaaaacaaaaaagacccataaaaatattttgaaatatgttatggctacatcacttgaaaattacattcatcatcttaaacgaaattattccaagttacagATGTCAACATGGTGGCAGATATTGGGAATACCATTGACTTTCTTCACAGAGTTCGTGCTCTTAAAATCAGGGGACAGAAAGGATATGTTGGAACATCGAATATCGTTTGGAATTCACTGGATATGAGTCTCTATTTTTGCAAAGGACTGAAAGCATTATGGgtatgttcaagattttgaaaaatgtactttataaatacctattctaataataaaaaccttttttaaaatcaaattggtaatcaatttcagATCGCCGACAGTGATGTATGTCGTATTAATGGAATCAAAAGTATAAGAGAGACACTTCGTCGTCTTGTCGTTCACTATTCGATGAAAAAGATCAAGGATTTGCTCTTCGATGAAGAAGATTTGGAAACTGGAATGCTTGTGGAGGAGATGGACACGTGGAAATGTCTGGAAGAAGT |
| Mutant Sequence |
>syb767(-873bp+1bp)
CCAATCGAGTTGCATGCGATTACCGAACGA-TGATGGACACGTGGAAATGTCTGGAAGAAGT |
| Phenotype |