| Strain Name | PHX5421 |
| Genotype | ins-3(syb5421) |
| Description | syb5421 is a SL2-GFP-H2B knock-in inserted after the termination codon of endogenous ins-3 gene on N2 by CRISPR/Cas9 system.
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| Wild type Sequence | >Wild type
ATAACTACTAAGTGCTGCCCTTCAAGATGA*gagtgctgtggctggaaaatagggaaacga |
| Mutant Sequence |
>syb5421:
ATAACTACTAAGTGCTGCCCTTCAAGATGAgctgtctcatcctactttcacctagttaactgcttgtcttaaaatctatgcttctctttagtatctaaaattttcctagaagcttacaagtatataaatggtctcttctcaataaaggttgtatatttattcatcttattgaatctgccatttcctcgtttttgcgagtttatataccttccaattttctttctattgtattttcaacttctaattttaattcagggaaactgcttcaacgcatcATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAACCACCAAAGCCATCTGCCAAGGGAGCCAAGAAGGCCGCCAAGACCGTTACGAAGCCAAAGGACGGAAAGAAGAGACGTCATGCCCGTAAGGAATCATACTCCGTCTACATCTACCGTGTCCTCAAGCAAGTTCATCCAGACACTGGAGTTTCCTCCAAAGCCATGTCTATCATGAACTCTTTTGTCAACGATGTCTTCGAGCGTATTGCTGCTGAAGCATCCCGTCTTGCTCACTACAACAAGCGTTCCACAATCTCATCCCGCGAAATTCAGACCGCTGTCCGTCTGATCCTTCCAGGAGAGCTTGCCAAGCACGCCGTGTCTGAGGGAACCAAGGCCGTTACCAAGTACACTTCCAGCAAGtAGgagtgctgtggctggaaaatagggaaacga |
| Phenotype |