| Strain Name | PHX1452 |
| Genotype | hlh-30(syb1452) |
| Description | syb1452 is a GSTGS-3Xflag-GSTGS-eGFP knock-in inserted before the termination codon of endogenous hlh-30 gene on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type
TCAAACTCGGGTCATTATCACATGGACTTTTCG*TAAactctctgcacctgcgccgccgttcct |
| Mutant Sequence |
>syb1452
TCTAACTCGGGTCATTATCACATGGACTTTTCGGGCAGTACCGGTAGCatgGATTACAAGGATCACGACGGAGACTATAAAGATCATGATATCGACTACAAGGATGATGATGATAAGGGCAGTACCGGTAGCATGTCCAAGGGAGAGGAGCTCTTCACCGGAGTCGTCCCAATCCTCGTCGAGCTCGACGGAGACGTCAACGGACACAAGTTCTCCGTCTCAGGAGAGGGAGAGGGAGACGCCACCTACGGAAAGCTCACCCTCAAGTTCATCTGCACCACCGGAAAGCTCCCAGTCCCATGGCCAACCCTCGTCACCACCTTCACTTACGGAGTCCAATGCTTCTCCCGTTACCCAGACCACATGAAGCGTCACGACTTCTTCAAGTCCGCCATGCCAGAGGGATACGTCCAAGAGCGTACCATCTTCTTCAAGgtaagtttaaacattaattaatactaactaaccctgattatttaaattttcagGACGACGGAAACTACAAGACCCGTGCCGAGGTCAAGTTCGAGGGAGACACCCTCGTCAACCGTATCGAGCTCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAATCGACTTCAAGGAGGACGGAAACATCCTCGGACACAAGCTCGAATACAACTACAACTCCCACAACGTCTACATCATGGCCGACAAGCAAAAGAACGGAATCAAGGTCAACTTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagATCCGTCACAACATCGAGGACGGATCTGTCCAACTCGCCGACCACTACCAACAAAACACCCCAATCGGAGACGGACCAGTCCTCCTCCCAGACAACCACTACCTCTCCACCCAATCCGCCCTCTCCAAGGACCCAAACGAGAAGCGTGACCACATGGTCCTCAAGGAGTTCGTCACCGCTGCCGGAATCACCCACGGAATGGACGAGCTCTACAAGTAAactctctgcacctgcgccgccgttcct |
| Phenotype |