| Strain Name | PHX784 |
| Genotype | ahcy-1(syb784 syb646) |
| Description | syb646 is a GFP knock-in inserted after the initiation codon of endogenous ahcy-1 gene on N2 by CRISPR/Cas9 system.
syb784 is the 169th aa of the T07A9.6.1(daf-18) protein mutate from C to S on syb646 strain by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type1
aattatttcaggtaaaacacattcgcaATG*GCCCAGTCTAAGCCAGCTTACAAGGTCGCT
>Wild type2
GGTGATCTTACCAACTTGGTTCACGCCAAGTACCCACAATACCTCGCTGGAATCCGCGGACTT |
| Mutant Sequence |
>syb646
AattatttcaggtaaaacacattcgcaATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGCACAGTCTAAGCCAGCTTACAAGGTCGCT
>syb784
GGTGATCTTACCAACTTGGTGCATGCCAAATGTCCACAGTACCTCGCTGGAATCCGCGGACT |
| Phenotype |