| Strain Name | PHX5463 |
| Genotype | ins-6(syb5463) |
| Description | syb5463 is a SL2-GFP-H2B knock-in inserted after the termination codon of endogenous ins-6 gene on N2 by CRISPR/Cas9 system.
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| Wild type Sequence | >Wild type
CCACgtaagttgaattaaatgtttacataattcaattatttatttcttgaactttcagAAGAAGGAAAGGACATTGCGACTGAATGCTGCGGAAATCAGTGTTCTGATGACTACATAAGATCTGCTTGTTGTCCATGA*atttttacctttactccgatatcagtctct |
| Mutant Sequence |
>syb5463:
CCtCgtaagttgaattaaatgtttacataattcaattatttatttcttgaactttcagAAGAAGGAAAGGACATTGCGACTGAATGTTGCGGAAATCAGTGTTCTGATGACTACATAAGATCTGCTTGTTGTCCATGAgctgtctcatcctactttcacctagttaactgcttgtcttaaaatctatgcttctctttagtatctaaaattttcctagaagcttacaagtatataaatggtctcttctcaataaaggttgtatatttattcatcttattgaatctgccatttcctcgtttttgcgagtttatataccttccaattttctttctattgtattttcaacttctaattttaattcagggaaactgcttcaacgcatcATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAACCACCAAAGCCATCTGCCAAGGGAGCCAAGAAGGCCGCCAAGACCGTTACGAAGCCAAAGGACGGAAAGAAGAGACGTCATGCCCGTAAGGAATCATACTCCGTCTACATCTACCGTGTCCTCAAGCAAGTTCATCCAGACACTGGAGTTTCCTCCAAAGCCATGTCTATCATGAACTCTTTTGTCAACGATGTCTTCGAGCGTATTGCTGCTGAAGCATCCCGTCTTGCTCACTACAACAAGCGTTCCACAATCTCATCCCGCGAAATTCAGACCGCTGTCCGTCTGATCCTTCCAGGAGAGCTTGCCAAGCACGCCGTGTCTGAGGGAACCAAGGCCGTTACCAAGTACACTTCCAGCAAGtGAatttttacctttactccgatatcagtctct |
| Phenotype |