Strain Name | PHX3072 |
Genotype | rab-3(syb3072) |
Description | syb3072 is a T2A::3XNLS::GFP knock-in inserted before the termination codon of endogenous rab-3 gene on N2 by CRISPR/Cas9 system. |
Wild type Sequence | >Wild type
CCAAAAGGACAGAAGCTCGAAGCGAATCCGACCCAAAAGCCTGCTCAACAGCAATGCAATTGC*TAAaaattctacgttctattatactatcta |
Mutant Sequence |
>syb3072:
CAACCTAAAGGACAGAAGCTCGAAGCGAATCCGACCCAGAAGCCTGCTCAACAGCAATGCAATTGCgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaACCGCTCCAAAGAAGAAACGCAAAGTACCGAGCTCAGAAAAAATGACGGCACCTAAAAAGAAACGCAAAGTACCGGTCGCCGAGAAGACAGCCCCAAAAAAGAAGCGTAAAGTTCCACGCGTCATGGGAgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagCGCGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGTAAaaattctacgttctattatactatcta |
Phenotype |