| Strain Name | PHX1772 |
| Genotype | pod-2(syb1772) |
| Description | syb1772 is a GGGGG-10xHis tag knock-in inserted before the termination codon of endogenous pod-2 gene on N2 by CRISPR/Cas9 system..
|
| Wild type Sequence | >Wild type
AAGCCGTGCCTCGCAACAGCGATGACATGGGTTGAAACGAATCTCCGATCATATGTGCAACCGAACAGCTCAATGGACGAGCAATACACACAAATTGAGCAGTATTACGAGACGCAGTTTGTCGATGATCTTGTCATTGCGATTAATGAGGATCGGAAACGgtaaattttcagggttttggctgaaaattggctgaaatttcggtgaaaaaccgacaattttcagcaataaattcatagaaattattgatttttaagcctaaaatttgtattttttcagATATGAACAGCAGCTCAACACGTTTATGAACAAAATTTCTTCGAAGCGACGA*TAAtaattttaactcgaatttttccccatt |
| Mutant Sequence |
>syb1772
AAACCGTGTCTCGCAACAGCGATGACATGGGTTGAAACGAATCTCCGATCATATGTGCAACCGAACAGCTCAATGGACGAGCAATACACACAAATTGAGCAGTATTACGAGACGCAGTTTGTCGATGATCTTGTCATTGCGATTAATGAAGATCGGAAACGgtaaattttcagggttttggctgaaaattggctgaaatttcggtgaaaaaccgacaattttcagcaataaattcatagaaattattgatttttaagcctaaaatttgtattttttcagATATGAACAGCAGCTCAACACGTTTATGAACAAAATTTCTTCGAAGCGcCGcGGAGGAGGAGGAGGACACCATCACCATCACCACCACCACCACCACTAAtaattttaactcgaatttttccccatt |
| Phenotype |