Strain Name | PHX5500 |
Genotype | ceh-44(syb5500) |
Description | syb5500 is a oxGFP knock-in inserted before the termination codon of endogenous ceh-44 gene on N2 by CRISPR/Cas9 system. |
Wild type Sequence | >Wild type
CATCAACATATGCTGGACAATCATAAGCAA*TAAttttttggatttgaattttttttattt |
Mutant Sequence |
>syb5500 CATCAACACATGtTaGACAATCATAAGCAAATGGTCTCCAAGGGAGAGGAGCTCTTCACCGGAGTCGTCCCAATCCTCGTCGAGCTCGACGGAGACGTCAACGGACACAAGTTCTCCGTCCGTGGAGAGGGAGAGGGAGACGCCACCAACGGAAAGCTCACCCTCAAGTTCATCTCCACCACCGGAAAGCTCCCAGTCCCATGGCCAACCCTCGTCACCACCCTCACCTACGGAGTCCAATCCTTCTCCCGTTACCCAGACCACATGAAGCGTCACGACTTCTTCAAGTCCGCCATGCCAGAGGGATACGTCCAAGAGCGTACCATCTCCTTCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGACGACGGAACCTACAAGACCCGTGCCGAGGTCAAGTTCGAGGGAGACACCCTCGTCAACCGTATCGAGCTCAAGGGAATCGACTTCAAGGAGGACGGAAACATCCTCGGACACAAGCTCGAGTACAACTTCAACTCCCACAACGTCTACATCACCGCCGACAAGCAAAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagAACGGAATCAAGGCCAACTTCAAGATCCGTCACAACGTCGAGGACGGATCCGTCCAACTCGCCGACCACTACCAACAAAACACCCCAATCGGAGACGGACCAGTCCTCCTCCCAGACAACCACTACCTCTCCACCCAATCCGTCCTCTCCAAGGACCCAAACGAGAAGCGTGACCACATGGTCCTCCTCGAGTTCGTCACCGCCGCCGGAATCACCCACGGAATGGACGAGCTCTACAAGTAAttttttggatttgaattttttttattt |
Phenotype |