Strain Name | PHX1049 |
Genotype | vha-13(syb1049) |
Description | syb1049 is a GFP-GRRIPGLIN knock-in inserted before the initiation codon of endogenous vha-13 gene on N2 by CRISPR/Cas9 system.
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Wild type Sequence | >Wild type
gctttctaaattcattcattccaggaaaag*ATGGCCGCAGAATCTTCGTACGGATTCGTTTACGGAGTGTCC |
Mutant Sequence |
>syb1049
gctttctaaattcattcattccaggaaaagATGTCAAAAGGAGAAGAGTTGTTTACTGGCGTGGTCCCTATCCTTGTTGAACTGGATGGTGACGTAAACGGACATAAGTTCTCTGTCAGTGGTGAGGGTGAAGGCGATGCTACCTACGGTAAATTAACTTTGAAGTTCATATGTACTACGGGAAAATTGCCTGTGCCTTGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACCTTGGTCACGACATTTTGTTACGGAGTCCAGTGCTTTTCGCGTTACCCGGATCATATGAAAAGACACGACTTTTTTAAGTCGGCCATGCCAGAAGGCTATGTACAAGAAAGAACCATATTCTTCAAAGATGACGGTAACTACAAAACGAgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGAGCAGAAGTTAAGTTCGAAGGTGACACTCTTGTAAACAGAATCGAACTTAAGGGTATTGATTTCAAAGAGGATGGAAATATATTAGGTCACAAACTTGAGTACAACTACAACTCTCACAATGTGTATATTATGGCTGATAAACAGAAGAATGGAATTAAGGTGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTTAAGATACGTCATAACATAGAGGACGGAAGTGTTCAATTAGCCGACCACTATCAACAAAATACACCTATTGGCGACGGACCGGTTCTGCTTCCAGACAATCACTACCTGTCGACACAAAGTGCCCTGAGTAAAGATCCTAACGAGAAGCGAGACCACATGGTACTGTTAGAGTTCGTGACCGCAGCCGGCATAACTCACGGAATGGACGAGTTGTACAAAGGACGTCGTATCCCAGGACTCATCAACATGGCCGCAGAATCTTCGTACGGATTCGTGTACGGAGTGTCG |
Phenotype |