| Strain Name | PHX1464 |
| Genotype | flp-11(syb1464) |
| Description | syb1464 is a SL2-egl-23cDNA(L229N)-GSGSGSGSG-mKate2 knock-in inserted after the termination codon of endogenous flp-11 gene on N2 by CRISPR/Cas9 system.
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| Wild type Sequence | >Wild type
GAACAACTCCTGAGGCGAATTCATCATTAA*aaatcatatgtttttctctctcacactctc |
| Mutant Sequence |
>syb1464
GAACAACTCCTGCGACGAATTCATCATTAAgctgtctcatcctactttcacctagttaactgcttgtcttaaaatctatgcttctctttagtatctaaaattttcctagaagcttacaagtatataaatggtctcttctcaataaaggttgtatatttattcatcttattgaatctgccatttcctcgtttttgcgagtttatataccttccaattttctttctattgtattttcaacttctaattttaattcagggaaactgcttcaacgcatcATGAAGCTCACGTTGAAGAAATCCGTATTCTCAAGGGATAAACATATCTTGCAAAAGGCGACACCACTATTCGTTCACTTTCTAATGATAGTAAGTGTGGGTGCCTACGCAATATTTGGAGCACTTGTAATGAGAAGTCTTGAATCGAGAACTGTCACAAGTATTGAAAAGAAGACGGATGTTCACAGAAGACATGTTAATTTGACTAATTTTCAACATCCACCAACTCCAATCACATTAGAGCAAAGACATCGACGGAGACGCAGACATAATGAGACAGCTTTGGAAGACCATTTGTCTGAAAAATTATCCAGAGAAAAACGTGCAGCAGCGCATATCATGAGAAGTCGAAAATGCGTTATCAGTGTGATAAAGAAAATGTCAAGCATGGAATGTTCATTTGACACTCTCGACGAGAAGCTCGTAAAAGCACTCGATGAATGTTACCACGTGGCAGTTGAACATAATACTCATGTGAATCATGTACTTTTCACGAATAGTAAGGAAGAAGTGGAGTCAGTTGGGGAAGAAGCAGAAGAAGATGTTTCCGAATGGTCATTTATGGACTCGTTGTTGTTTGCATTCACTGTTATTACGACGATTGGATACGGAAACGTGGCACCTCGAACATTTGGTGGCCGTCTATTTGTCATTGGTTATGGTCTAATTGGTATTCCGTTTACAAACCTGGCAATTGCAGATCTCGGAAAATTCATATCAGAAATGATGGTGGAGGCGAAAAGTTTTTGTAGGAAAACCTGGAAAAAACTCAAAAAAGCGTGGAACCCGAATTTCATTCGCGCAAAGGATCTTTCAAATACGGATATTGAGGAGAAAATATTGGATAATGAGAAAATCGAAAATGAGCCGGAAACTTCAGAAGTATCAGAAGAAGAAGACGATTTGACAGAGACAGAAGCCACGTCACTTTTCATTTTATTTTTGGTTTATATCGCATTTGGAGGGTTCATGTTAGCTGCTTATGAACCTGATATGGACTTTTTCAAAGCGGTCTACTTTAATTTTGTGACATTGACATCAATTGGTCTGGGAGATATTGTACCGAGAAGTGAAACCTATATGCTCATTACAATAGTCTACATTGCAATTGGTCTTGCTCTTACCACAATTGCCATTGAAATCGCCGCAGATGCATTGAAGAAGCTGCATTATTTTGGAAGAAAAATCGAAAATGTTGGAAATGTGGCTATATGGTTTGGAGGAAAGAAAATTACAATGAAAGCACTGGTCAAAAACCTCGGTGACCAGTTCAATCTTCCAACTACCGTAGTCAAGAATTTGAATTTGGATCATTTTGTGGATCAAGCGATTAAAGTGGAGGAAGGAGAGATTGAGACACTCAGACCGCCGCCTTATGAGCCACCGTCTGATCGATTTGAAGCTGAATTCGCTGATGAACCAGAATGTGAATGGATCCGTGATCCGACTCCAACTCCACCCCCATCACCTCAACCGGTTTATCGTCTTCCATCTCCAAAACCAGTTACACCGGAACCTCTACCAAGTCCAACAATAACTGATGTATCACTTGCGATTGCAACACCATCACCTGAAGAATCGGATGATGATCAAGAACTAATTCTCCCATCACCTGAACCGAGTCCAGTTCGAGAGCCAACTCCACCACCGCCTCCACGGGAGCCAACACCTCGTGAGCCAACTCCTGAGCCAGAGCCAGTTCGAGAGCCGACGCCTCCACCTCCGCCACCTGCCAAGCCCCGTCCACTGACTGCCGCTGAAATCGCGGCTCAAAAACGCAAAGCGTACAGCGAAGAAGCATGGCGTCGATACCAAGAATACCAGAAACAATGGAAGAAGTTCCGTCAAACTCAGAAAACTCCCGCACCATCTGGAGCCTCTACATCAGGGGCATCAACATCAAAACCGTCTGGAACATCACCGGAAAGTGGAGCCGGAGTATGTACTGGACCATCAACAAGGAGTCAATCAATAACATCAGTTGCATCTGGAAAGACATCAAGAAGTGCAACACCGGAAAGCAAGAAATCATCACATTTGAGTGGCTCATCGAGAAGAGAAAGCGGTGGAAAAGGATCCGGATCCGGATCCGGATCCGGAATGTCCGAGCTCATCAAGGAGAACATGCACATGAAGCTCTACATGGAGGGAACCGTCAACAACCACCACTTCAAGTGCACCTCCGAGGGAGAGGGAAAGCCATACGAGGGAACCCAAACCATGCGTATCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGCCGTCGAGGGAGGACCACTCCCATTCGCCTTCGACATCCTCGCCACCTCCTTCATGTACGGATCCAAGACCTTCATCAACCACACCCAAGGAATCCCAGACTTCTTCAAGCAATCCTTCCCAGAGGGATTCACCTGGGAGCGTGTCACCACCTACGAGGACGGAGGAGTCCTCACCGCCACCCAAGACACCTCCCTCCAAGACGGATGCCTCATCTACAACGTCAAGATCCGTGGAGTCAACTTCCCATCCAACGGACCAGTCATGCAAAAGAAGACCCTCGGATGGGAGGCCTCCACCGAGACCCTCTACCCAGCCGACGGAGGACTCGAGGGACGTGCCGACATGGCCCTCAAGCTCGTCGGAGGAGGACACCTCATCTGCAACCTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCACCTACCGTTCCAAGAAGCCAGCCAAGAACCTCAAGATGCCAGGAGTCTACTACGTCGACCGTCGTCTCGAGCGTATCAAGGAGGCCGACAAGGAGACCTACGTCGAGCAACACGAGGTCGCCGTCGCCCGTTACTGCGACCTCCCATCCAAGCTCGGACACCGTTAAaaatcatatgtttttctctctcacactctc |
| Phenotype |