Strain Name | PHX1805 |
Genotype | ser-1(syb1805) |
Description | syb1805 is a T2aPeptide-mNeonGreen knock-in inserted before the termination codon of endogenous ser-1 gene on N2 by CRISPR/Cas9 system.
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Wild type Sequence | >Wild type
TTTGATAGTGCCATCAAGGAAACATTCTTG*TAAattcaaaagccatgtggctcttcatat |
Mutant Sequence |
>syb1805:
TTTGATAGTGCCATCAAAGAAACATTCTTGgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaGTCTCCAAGGGAGAGGAGGACAACATGGCCTCCCTCCCAGCCACCCACGAGCTCCACATCTTCGGATCCATCAACGGAGTCGACTTCGACATGGTCGGACAAGGAACCGGAAACCCAAACGACGGATACGAGGAGCTCAACCTCAAGTCCACCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGGAGACCTCCAATTCTCCCCATGGATCCTCGTCCCACACATCGGATACGGATTCCACCAATACCTCCCATACCCAGACGGAATGTCCCCATTCCAAGCCGCCATGGTCGACGGATCCGGATACCAAGTCCACCGTACCATGCAATTCGAGGACGGAGCCTCCCTCACCGTCAACTACCGTTACACCTACGAGGGATCCCACATCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAGAGGCCCAAGTCAAGGGAACCGGATTCCCAGCCGACGGACCAGTCATGACCAACTCCCTCACCGCCGCCGACTGGTGCCGTTCCAAGAAGACCTACCCAAACGACAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCATCATCTCCACCTTCAAGTGGTCCTACACCACCGGAAACGGAAAGCGTTACCGTTCCACCGCCCGTACCACCTACACCTTCGCCAAGCCAATGGCCGCCAACTACCTCAAGAACCAACCAATGTACGTCTTCCGTAAGACCGAGCTCAAGCACTCCAAGACCGAGCTCAACTTCAAGGAGTGGCAAAAGGCCTTCACCGACGTCATGGGAATGGACGAGCTCTACAAGTAAattcaaaagccatgtggctcttcatat |
Phenotype |