| Strain Name | PHX5520 |
| Genotype | smc-3(syb5520) |
| Description | syb5520 is a GGGGS-AID-EmGFP knock-in inserted before the termination codon of endogenous smc-3 gene on N2 by CRISPR/Cas9 system..
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| Wild type Sequence | >Wild type
GATTTTGTTGAGGATGACACCACaCAtGGA*TAAatatatgcatattttgaatattttttt |
| Mutant Sequence |
>syb5520:
GATTTTGTTGAGGATGACACgACTCAtGGAgggggaggaggatcgATGCCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGCCCGGAGGCGGCGGCGTTCGTGAAGGTTTCTAAAGGAGAAGAACTTTTCACAGGAGTTGTTCCAATACTTGTTGAACTTGATGGAGATGTAAATGGACATAAGTTCTCTGTTTCTGGCGAAGGAGAAGGAGATGCTACTTATGGAAAACTTACTCTCAAGTTCATTTGCACTACTGGAAAACTTCCCGTTCCATGGCCAACTCTTGTTACTACTTTgACTTATGGAGTTCAATGTTTCGCTCGTTATCCAGACCATATGAAACAACACGATTTCTTCAAATCTGCTATGCCAGAAGGATATGTTCAAGAAAGAACTATTTTCTTCAAAGATGATGGAAACTACAAAACTCGTGCGGAAGTAAAGTTTGAGGGAGATACTCTTGTAAATAGAATAGAACTCAAAGGAATAGATTTCAAAGAAGATGGAAATATACTTGGACATAAACTTGAATATAACTACAATAGTCATAAAGTTTATATTACTGCTGATAAACAAAAAAATGGAATAAAAGTAAACTTCAAAACTCGTCATAATATAGAAGATGGAAGTGTTCAACTTGCTGACCATTACCAACAAAATACTCCAATAGGAGATGGCCCAGTTCTTCTCCCAGATAATCATTATCTTTCTACTCAATCTGCTCTTTCTAAAGACCCAAATGAAAAAAGAGACCATATGGTTCTTTTAGAGTTCGTTACTGCGGCGGGAATAACTCTTGGAATGGATGAACTTTACAAATAAatatatgcatattttgaatattttttt |
| Phenotype |