Strain Name | PHX1841 |
Genotype | egl-9(syb1841) |
Description | syb1841 is a wrmscarlet-AID-2xHA knock-in inserted before the termination codon of endogenous egl-9 gene on N2 by CRISPR/Cas9 system.
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Wild type Sequence | >Wild type
CCTCCTTCCACAAACCCAGAGTATTACATC*TGAagtttttctggtttttgttactttcta |
Mutant Sequence |
>syb1841
CCTCCTTCCACAAACCCAGAATATTACATCATGGTCAGCAAGGGAGAGGCAGTTATCAAGGAGTTCATGCGTTTCAAGGTCCACATGGAGGGATCCATGAACGGACACGAGTTCGAGATCGAGGGAGAGGGAGAGGGACGTCCATACGAGGGAACCCAAACCGCCAAGCTCAAGGTCACCAAGGGAGGACCACTCCCATTCTCCTGGGACATCCTCTCCCCACAATTCATGTACGGATCCCGTGCCTTCACCAAGCACCCAGCCGACATCCCAGACTACTACAAGCAATCCTTCCCAGAGGGATTCAAGTGGGAGCGTGTCATGAACTTCGAGGACGGAGGAGCCGTCACCGTCACCCAAGACACCTCCCTCGAGGACGGAACCCTCATCTACAAGGTCAAGCTCCGTGGAACCAACTTCCCACCAGACGGACCAGTCATGCAAAAGAAGACCATGGGATGGGAGGCCTCCACCGAGCGTCTCTACCCAGAGGACGGAGTCCTCAAGGGAGACATCAAGATGGCCCTCCGTCTCAAGGACGGAGGACGTTACCTCGCCGACTTCAAGACCACCTACAAGGCCAAGAAGCCAGTCCAAATGCCAGGAGCCTACAACGTCGACCGTAAGCTCGACATCACCTCCCACAACGAGGACTACACCGTCGTCGAGCAATACGAGCGTTCCGAGGGACGTCACTCCACCGGAGGAATGGACGAGCTCTACAAGATGCCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGCCCGGAGGCGGCGGCGTTCGTGAAGTACCCATATGATGTCCCGGATTACGCTTACCCATACGATGTTCCAGATTACGCTTGAagtttttctggtttttgttactttcta |
Phenotype |