Strain Name | PHX1849 |
Genotype | ceh-23(syb1849) |
Description | syb1849 is a GFP knock-in inserted before the termination codon of endogenous ceh-23 gene on N2 by CRISPR/Cas9 system.
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Wild type Sequence | >Wild type
GGCCTATCACCTTCTCAAGTTAGAATTTGGTTTCAAAACCGGAGAAGCAAACATCGTAGGAAACAACAAGAGGAGCAACAGAGCACGACGTTAGAAGAAAAATCTGAAGAAATTGGAAAAGATGAAGAGGAAGATGATGAAGAAGATGAAGATGATGTAAAAGTTCTGAAT*TAAtttaattttttttttcgtgtttacttg |
Mutant Sequence |
>syb1849
GGTCTATCACCTTCTCAGGTTAGAATTTGGTTTCAAAACCGGAGAAGCAAACATCGAAGGAAACAACAAGAGGAGCAACAGAGCACGACGTTAGAAGAAAAATCTGAAGAAATTGGAAAAGATGAAGAGGAAGATGATGAAGAAGATGAAGATGATGTAAAAGTTCTGAATAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAAtttaattttttttttcgtgtttacttg |
Phenotype |