| Strain Name | PHX1866 |
| Genotype | ser-4(syb1866) |
| Description | syb1866 is a T2A-mNeonGreen knock-in inserted before the termination codon of endogenous ser-4 gene on N2 by CRISPR/Cas9 system.
|
| Wild type Sequence | >Wild type
CGAATCATCAAACGGATGTGCCTTATTCACGATTAT*TGAttttttctcaatcccataaaatttcga |
| Mutant Sequence |
>syb1866:
CGCATCATCAAACGGATGTGCCTCATTCACGATTATGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGCCCAGTCTCCAAGGGAGAGGAGGACAACATGGCCTCCCTCCCAGCCACCCACGAGCTCCACATCTTCGGATCCATCAACGGAGTCGACTTCGACATGGTCGGACAAGGAACCGGAAACCCAAACGACGGATACGAGGAGCTCAACCTCAAGTCCACCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGGAGACCTCCAATTCTCCCCATGGATCCTCGTCCCACACATCGGATACGGATTCCACCAATACCTCCCATACCCAGACGGAATGTCCCCATTCCAAGCCGCCATGGTCGACGGATCCGGATACCAAGTCCACCGTACCATGCAATTCGAGGACGGAGCCTCCCTCACCGTCAACTACCGTTACACCTACGAGGGATCCCACATCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAGAGGCCCAAGTCAAGGGAACCGGATTCCCAGCCGACGGACCAGTCATGACCAACTCCCTCACCGCCGCCGACTGGTGCCGTTCCAAGAAGACCTACCCAAACGACAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCATCATCTCCACCTTCAAGTGGTCCTACACCACCGGAAACGGAAAGCGTTACCGTTCCACCGCCCGTACCACCTACACCTTCGCCAAGCCAATGGCCGCCAACTACCTCAAGAACCAACCAATGTACGTCTTCCGTAAGACCGAGCTCAAGCACTCCAAGACCGAGCTCAACTTCAAGGAGTGGCAAAAGGCCTTCACCGACGTCATGGGAATGGACGAGCTCTACAAGTGAttttttctcaatcccataaaatttcga |
| Phenotype |