Strain Name | PHX1884 |
Genotype | ceh-75(syb1884) |
Description | syb1884 is a GFP knock-in inserted before the termination codon of endogenous ceh-75 gene on N2 by CRISPR/Cas9 system.
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Wild type Sequence | >Wild type
CCGAAATACAGACACTTGCCGAAAAGTACAATATGACATGCAAGCAAATCAAGCAGTATTTCAGAAATTTAAGATTTCGGAGCAAGGGgtacgagtttttgctgtttttaatttaaaactaagactaatttcagACACGACTCAATGAAGATGAGCAGCAAA*TAAatcgtcaaaaatacacttttttgtttc |
Mutant Sequence |
>syb1884
CCGAAATACAGACTCTTGCCGAAAAGTACAATATGACATGCAAGCAAATCAAGCAGTATTTCAGAAATTTAAGATTTCGGAGCAAAGGgtacgagtttttgctgtttttaatttaaaactaagactaatttcagACACGACTCAATGAAGATGAGCAGCAAAAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAAatcgtcaaaaatacacttttttgtttc |
Phenotype |