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Strain Name
PHX1941
Genotype
ser-7(syb1941)
Description
syb1941 is a T2A-mNeonGreen knock-in inserted before the termination codon of endogenous ser-7 gene on N2 by CRISPR/Cas9 system.
Wild type Sequence
>Wild type CTTCTgtgagtataaccctacttatccatatatctcaactgttatatactacatcacgtttttaacttttctttcttctattcgagtgttacctttttcttctttttaaaagcatggagtttgcgattttatgcgattttttctcagtgttggtttgcgatttgcttccaaacattcaacgttttgcatgagttttacttctaaagtgaaatgattttgattctttgcacgtgacactgtttcaaaattgtacgtatgatattttcagATCTCGACGCAATGACAGTCACGAAGCAAGTGACGTC*TAGcacactacacattatgtaccatttcat
Mutant Sequence
>syb1941: TTGCTgtgagtataaccctacttatccatatatctcaactgttatatactacatcacgtttttaacttttctttcttctattcgagtgttacctttttcttctttttaaaagcatggagtttgcgattttatgcgattttttctcagtgttggtttgcgatttgcttccaaacattcaacgttttgcatgagttttacttctaaagtgaaatgattttgattctttgcacgtgacactgtttcaaaattgtacgtatgatattttcagATCTCGACGCAATGACAGTCACGAAGCAAGTGACGTCgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaGTCTCCAAGGGAGAGGAGGACAACATGGCCTCCCTCCCAGCCACCCACGAGCTCCACATCTTCGGATCCATCAACGGAGTCGACTTCGACATGGTCGGACAAGGAACCGGAAACCCAAACGACGGATACGAGGAGCTCAACCTCAAGTCCACCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGGAGACCTCCAATTCTCCCCATGGATCCTCGTCCCACACATCGGATACGGATTCCACCAATACCTCCCATACCCAGACGGAATGTCCCCATTCCAAGCCGCCATGGTCGACGGATCCGGATACCAAGTCCACCGTACCATGCAATTCGAGGACGGAGCCTCCCTCACCGTCAACTACCGTTACACCTACGAGGGATCCCACATCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAGAGGCCCAAGTCAAGGGAACCGGATTCCCAGCCGACGGACCAGTCATGACCAACTCCCTCACCGCCGCCGACTGGTGCCGTTCCAAGAAGACCTACCCAAACGACAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCATCATCTCCACCTTCAAGTGGTCCTACACCACCGGAAACGGAAAGCGTTACCGTTCCACCGCCCGTACCACCTACACCTTCGCCAAGCCAATGGCCGCCAACTACCTCAAGAACCAACCAATGTACGTCTTCCGTAAGACCGAGCTCAAGCACTCCAAGACCGAGCTCAACTTCAAGGAGTGGCAAAAGGCCTTCACCGACGTCATGGGAATGGACGAGCTCTACAAGTAGcacactacacattatgtaccatttcat
Phenotype