Strain Name | PHX1941 |
Genotype | ser-7(syb1941) |
Description | syb1941 is a T2A-mNeonGreen knock-in inserted before the termination codon of endogenous ser-7 gene on N2 by CRISPR/Cas9 system.
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Wild type Sequence | >Wild type
CTTCTgtgagtataaccctacttatccatatatctcaactgttatatactacatcacgtttttaacttttctttcttctattcgagtgttacctttttcttctttttaaaagcatggagtttgcgattttatgcgattttttctcagtgttggtttgcgatttgcttccaaacattcaacgttttgcatgagttttacttctaaagtgaaatgattttgattctttgcacgtgacactgtttcaaaattgtacgtatgatattttcagATCTCGACGCAATGACAGTCACGAAGCAAGTGACGTC*TAGcacactacacattatgtaccatttcat |
Mutant Sequence |
>syb1941:
TTGCTgtgagtataaccctacttatccatatatctcaactgttatatactacatcacgtttttaacttttctttcttctattcgagtgttacctttttcttctttttaaaagcatggagtttgcgattttatgcgattttttctcagtgttggtttgcgatttgcttccaaacattcaacgttttgcatgagttttacttctaaagtgaaatgattttgattctttgcacgtgacactgtttcaaaattgtacgtatgatattttcagATCTCGACGCAATGACAGTCACGAAGCAAGTGACGTCgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaGTCTCCAAGGGAGAGGAGGACAACATGGCCTCCCTCCCAGCCACCCACGAGCTCCACATCTTCGGATCCATCAACGGAGTCGACTTCGACATGGTCGGACAAGGAACCGGAAACCCAAACGACGGATACGAGGAGCTCAACCTCAAGTCCACCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGGAGACCTCCAATTCTCCCCATGGATCCTCGTCCCACACATCGGATACGGATTCCACCAATACCTCCCATACCCAGACGGAATGTCCCCATTCCAAGCCGCCATGGTCGACGGATCCGGATACCAAGTCCACCGTACCATGCAATTCGAGGACGGAGCCTCCCTCACCGTCAACTACCGTTACACCTACGAGGGATCCCACATCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAGAGGCCCAAGTCAAGGGAACCGGATTCCCAGCCGACGGACCAGTCATGACCAACTCCCTCACCGCCGCCGACTGGTGCCGTTCCAAGAAGACCTACCCAAACGACAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCATCATCTCCACCTTCAAGTGGTCCTACACCACCGGAAACGGAAAGCGTTACCGTTCCACCGCCCGTACCACCTACACCTTCGCCAAGCCAATGGCCGCCAACTACCTCAAGAACCAACCAATGTACGTCTTCCGTAAGACCGAGCTCAAGCACTCCAAGACCGAGCTCAACTTCAAGGAGTGGCAAAAGGCCTTCACCGACGTCATGGGAATGGACGAGCTCTACAAGTAGcacactacacattatgtaccatttcat |
Phenotype |