| Strain Name | PHX6035 |
| Genotype | wapl-1(syb6035) |
| Description | syb6035 is a GGGGS-degron-EmGFP knock-in inserted before the termination codon of endogenous wapl-1 gene on N2 by CRISPR/Cas9 system..
|
| Wild type Sequence | >Wild type
tcgGGATTCcGgGCTATCGAGCGTGTGATTGAGTACcTcGAGCGGCTCGAG*TGAtttaatgtttatttcttgattcttttt |
| Mutant Sequence |
>syb6035:
AGTGGATTCAGAGCTATCGAGCGTGTGATTGAGTACTTAGAGCGGCTCGAGgggggaggaggatcgATGCCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGCCCGGAGGCGGCGGCGTTCGTGAAGGTTTCTAAAGGAGAAGAACTTTTCACAGGAGTTGTTCCAATACTTGTTGAACTTGATGGAGATGTAAATGGACATAAGTTCTCTGTTTCTGGCGAAGGAGAAGGAGATGCTACTTATGGAAAACTTACTCTCAAGTTCATTTGCACTACTGGAAAACTTCCCGTTCCATGGCCAACTCTTGTTACTACTTTgACTTATGGAGTTCAATGTTTCGCTCGTTATCCAGACCATATGAAACAACACGATTTCTTCAAATCTGCTATGCCAGAAGGATATGTTCAAGAAAGAACTATTTTCTTCAAAGATGATGGAAACTACAAAACTCGTGCGGAAGTAAAGTTTGAGGGAGATACTCTTGTAAATAGAATAGAACTCAAAGGAATAGATTTCAAAGAAGATGGAAATATACTTGGACATAAACTTGAATATAACTACAATAGTCATAAAGTTTATATTACTGCTGATAAACAAAAAAATGGAATAAAAGTAAACTTCAAAACTCGTCATAATATAGAAGATGGAAGTGTTCAACTTGCTGACCATTACCAACAAAATACTCCAATAGGAGATGGCCCAGTTCTTCTCCCAGATAATCATTATCTTTCTACTCAATCTGCTCTTTCTAAAGACCCAAATGAAAAAAGAGACCATATGGTTCTTTTAGAGTTCGTTACTGCGGCGGGAATAACTCTTGGAATGGATGAACTTTACAAATGAtttaatgtttatttcttgattcttttt |
| Phenotype |