| Strain Name | PHX2193 |
| Genotype | flp-11b(syb2193) |
| Description | syb2193 is a SL2(gpd-2)-mKate2(two introns)-linker(GSGSGSGSG)-twk-18(e1913)(one intron) knock-in inserted after the termination codon of endogenous flp-11 gene on N2 by CRISPR/Cas9 system..
twk-18(e1913) was knocked into the endogenous locus of flp-11 to express a potassium channel in RIS that causes very strong inactivation of RIS.
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| Wild type Sequence | >Wild type
GAACAACTCCTGAGGCGAATTCATCATTAA*aaatcatatgtttttctctctcacactctc |
| Mutant Sequence |
>syb2193:
GAACAACTCCTGCGACGAATTCATCATTAAgctgtctcatcctactttcacctagttaactgcttgtcttaaaatctatgcttctctttagtatctaaaattttcctagaagcttacaagtatataaatggtctcttctcaataaaggttgtatatttattcatcttattgaatctgccatttcctcgtttttgcgagtttatataccttccaattttctttctattgtattttcaacttctaattttaattcagggaaactgcttcaacgcatcaaaaATGTCCGAGCTCATCAAGGAGAACATGCACATGAAGCTCTACATGGAGGGAACCGTCAACAACCACCACTTCAAGTGCACCTCCGAGGGAGAGGGAAAGCCATACGAGGGAACCCAAACCATGCGTATCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGCCGTCGAGGGAGGACCACTCCCATTCGCCTTCGACATCCTCGCCACCTCCTTCATGTACGGATCCAAGACCTTCATCAACCACACCCAAGGAATCCCAGACTTCTTCAAGCAATCCTTCCCAGAGGGATTCACCTGGGAGCGTGTCACCACCTACGAGGACGGAGGAGTCCTCACCGCCACCCAAGACACCTCCCTCCAAGACGGATGCCTCATCTACAACGTCAAGATCCGTGGAGTCAACTTCCCATCCAACGGACCAGTCATGCAAAAGAAGACCCTCGGATGGGAGGCCTCCACCGAGACCCTCTACCCAGCCGACGGAGGACTCGAGGGACGTGCCGACATGGCCCTCAAGCTCGTCGGAGGAGGACACCTCATCTGCAACCTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCACCTACCGTTCCAAGAAGCCAGCCAAGAACCTCAAGATGCCAGGAGTCTACTACGTCGACCGTCGTCTCGAGCGTATCAAGGAGGCCGACAAGGAGACCTACGTCGAGCAACACGAGGTCGCCGTCGCCCGTTACTGCGACCTCCCATCCAAGCTCGGACACCGTGGATCCGGATCCGGATCCGGATCCGGAATGGCCATCGTCGCCCAAGGAGTCTCCACCATCCTCACCACCTTCCAAAAGACCTTCAAGGGACTCCTCCCACTCATCATCCTCGTCGCCTACACCCTCCTCGGAGCCTGGATCTTCTGGATGATCGAGGGAGAGAACGAGCGTGAGATGCTCATCGAGCAACAAAAGGAGCGTGACGAGCTCATCCGTCGTACCGTCTACAAGATCAACCAACTCCAAATCAAGCGTCAACGTCGTCTCATGACCGCCGAGGAGGAGTACAACCGTACCGCCAAGGTCCTCACCACCTTCCAAGAGACCCTCGGAATCGTCCCAGCCGACATGGACAAGGACATCCACTGGACCTTCCTCGGATCCATCTTCTACTGCATGACCGTCTACACCACCATCGGATACGGAAACATCGTCCCAGGAACCGGATGGGGACGTTTCGCCACCATCCTCTACGCCTTCATCGGAATCCCACTCACCGTCCTCTCCCTCTACTGCCTCGACTCCCTCTTCGCCAAGGGATGCAAGATGCTCTGGCGTTTCTTCCTCAAGTCCACCCGTGTCGTCTCCAAGGACCTCTCCAACAAGATCTCCGAGGCCGCCGACAACATCGAGGAGGGAACCACCGCCATCACCCCATCCGCCGAGAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagACCGAGAACAACGACGACGACCTCCTCTCCTTCCCAATCTCCGGACTCCTCCTCATCACCGTCATCTGGGTCATCTTCTGCGCCGTCCTCTTCACCTTCCTCGAGGAGTGGGACTTCGGAACCTCCCTCTACTTCACCCTCATCTCCTTCACCACCATCGGATTCGGAGACATCCTCCCATCCGACTACGACTTCATGCCAATCGTCGGAGTCCTCCTCCTCATCGGACTCTCCCTCGTCTCCACCGTCATGACCCTCATCCAACAACAAATCGAGGCCCTCGCCTCCGGAATGAAGGACAACATCGACCAAGAGTACGCCCGTGCCCTCAACGAGGCCCGTGAGGACGGAGAGGTCGACGAGCACGTCGACCCAGAGGAGGACCCAGAGAACAACAAGAAGTCCTTCGACGCCGTCATCTCCCGTATGAACTGGTCCAAGCGTGGACTCTACTACCTCCTCCCAGACTCCCAAAAGAAGGAGCTCGCCAAGCAATCCGAGAAGAAGATGGGACGTAAGTCCATCAAGATCCAAACCGACAACGACCTCCTCGAGACCCTCATCCGTGAGGAGATCCTCAAGGCCGAGCTCAACAACGAGATGCACAAGTACACCGCCCCACGTTCCTCCCACCAACCAAAGCTCGTCTACTCCGACGTCCGTGAGAAGGAGGTCCCAATCGAGGTCGTCCGTGTCGAGCACTTCAACCACGGAAACGAGGACTACCTCGAGCACGACATCTAAaaatcatatgtttttctctctcacactctc |
| Phenotype |