你的位置:
Strain Name
PHX2307
Genotype
ceh-13(syb2307)
Description
syb2307 is a GASGASGAS-mNenogreen-GASGASGAS-3XFLAG-GASGASGAS-AID knock-in inserted before the termination codon of endogenous ceh-13 gene on N2 by CRISPR/Cas9 system.
Wild type Sequence
>Wild type TCATGCAGTGGAGAAGATGTCAAAAACTTCAAG*TAAtccatccgtctccaatctatttttata
Mutant Sequence
>syb2307: TCTTGCAGTGGAGAAGATGTCAAAAACTTCAAGGGAGCATCGGGAGCCTCAGGAGCATCGATGGTCTCCAAGGGAGAGGAGGACAACATGGCCTCCCTCCCAGCCACCCACGAGCTCCACATCTTCGGATCCATCAACGGAGTCGACTTCGACATGGTCGGACAAGGAACCGGAAACCCAAACGACGGATACGAGGAGCTCAACCTCAAGTCCACCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGGAGACCTCCAATTCTCCCCATGGATCCTCGTCCCACACATCGGATACGGATTCCACCAATACCTCCCATACCCAGACGGAATGTCCCCATTCCAAGCCGCCATGGTCGACGGATCCGGATACCAAGTCCACCGTACCATGCAATTCGAGGACGGAGCCTCCCTCACCGTCAACTACCGTTACACCTACGAGGGATCCCACATCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGGAGAGGCCCAAGTCAAGGGAACCGGATTCCCAGCCGACGGACCAGTCATGACCAACTCCCTCACCGCCGCCGACTGGTGCCGTTCCAAGAAGACCTACCCAAACGACAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCATCATCTCCACCTTCAAGTGGTCCTACACCACCGGAAACGGAAAGCGTTACCGTTCCACCGCCCGTACCACCTACACCTTCGCCAAGCCAATGGCCGCCAACTACCTCAAGAACCAACCAATGTACGTCTTCCGTAAGACCGAGCTCAAGCACTCCAAGACCGAGCTCAACTTCAAGGAGTGGCAAAAGGCCTTCACCGACGTCATGGGAATGGACGAGCTCTACAAGGGAGCATCGGGAGCCTCAGGAGCATCGGATTATAAAGACGATGACGATAAGCGTGACTACAAGGACGACGACGACAAGCGTGATTACAAGGATGACGATGACAAGGGAGCATCGGGAGCCTCAGGAGCATCGatgcctaaagatccagccaaacctccggccaaggcacaagttgtgggatggccaccggtgagatcataccggaagaacgtgatggtttcctgccaaaaatcaagcggtggcccggaggcggcggcgttcgtgaagTAAtccatccgtctccaatctatttttata
Phenotype