| Strain Name | PHX2493 |
| Genotype | lgc-38(syb2346 syb2493) |
| Description | syb2346 is Pflp-11::dpy-10Crispr site::flp-11b-3’utr inserted at the 5'UTR of endogenous lgc-38 gene on N2 strain by CRISPR/Cas9 system..
syb2493 is a gene replacement editing with dpy-10 Crispr site deletion (31bp) and ReaChR-linker-mKate2 insertion on syb2346 strain by CRISPR/Cas9 system. |
| Wild type Sequence | >syb2346-Wild type
gttaaataaatgaggtatatagttaaataa*acgaggtatatgggcttcagatcttcaact
>syb2493-Wild type
cactttttgcagttcatactgaataaaaaaGTACCGCTCGTGGTGCCTATGGTAGCGGAGCaaatcatatgtttttctctctcacactctc |
| Mutant Sequence |
>syb2346:
gttaaataaatgaggtatatagttaaataacacaccagatgatgtgagtgggcctgggtcgagctgcacattttggagaaacccaaaaaccgtgtttcataagtttgaatacaatgactttgtttttttgaaggatttttgtgcagcgttgttttaaaaaacggttttcataagatttttatcacagttgaactcctatatttcttccctcactaattcttgatgtgtggacaaaactgtaagtctgatcaaggcaacaacacaacatagaccacttttctagtccagtaaaaaatcgaactctgatattcagactggtatcacttcttagagtcagaagatggtacagcttgggaaaaacttggaaaaactatcccagactgccaaaactgaagcttaaagtgagcacaaaattgctccaaactttttttgcagctcacattcggtccaagtgttgttcaatttatgatgattatcagtctgttttgccaaattcggatccaactttttttttttttttaggagaatttattttaaagctcgccattgaaatacatgtaaaaatctaagcttttaaatttaacagaaaatgagattatcttttttggaatgaaacctagaattttcaaaataactagcatctcaaaataactagcatccaagatgctctttgttttcataaattttagttgtttccagtcccgaaaaaaaatttcaatcatttccttaggttcaataagtgttttgtctggtctcttgttgcaatttttgaaaaaggaataacattgtgctcagactgaaaaatgaaaaaagatgatgtaagttggtctaggctttaattccagtttccgttccactagtgatcttatcatttattcatatcaagtttcatttgatgttcgattcattgtaggtttcattgttccctctgactattcatcaagttctatgaaaccattgtggcttactagttccccaaagttggaaagatctggagaaagaaaaacaagtatcccaaaagcccaaaccttttttttccaaatgttgagcctctaaagttgatgaatggggtgagagaggttttcatggttttatggcccatcagttccagtagagttcctcatatcttttcgcacttcatttttcaatctgttaagagtaattcccccacaacttttagcatcctcaatcctggttccacccacaaacacccacactccattgaatataagattataaaatataagttaaaatacgaaaagatgcaaaactgtgacgacgaaacttaacaacctgctgacgtgtcaaatatttccgcatcttaccttcgtgggcatcttctaactcataaatttatgatgttcgaatcccggcgccaagtgatgtaaactcatttaatatttgaggaaaaagcatagacgaatccattttgcatctaatttatttttctagtttcacacccatgtttcgcttggcataatcagtgtttgagcgcactttttgcaaggtgcgtcttcttgtaaagaaagattttcaaaaaagtgcaaaagtgggggagattgaaaaacgataagaaggtttcatgtcaaacaaggacacacgaatatataaatacgggtcatttttcaaactttccattgtgactttttggaatatttttttcgtaaaaatgaaatcgattttctgaccaatatctcaactttaaaacttctaattctgctttttttcaatcttctgtaaacaggagtgcgtcgaaatttcggcagtttcggcatattgccaaaagtttatagtccaacaattgccggaactggtgatttagctaaacaataaacctcaaaatattaggtatttcagtaagaaaatgacctattgattatgactttgaatcaagttttgatatatttttaatttttgaaatatgatgaaaattaaaaattatcaaaaattctggcaattttggcacatcggcacacaatgcgaaatttgcggcaactgccgatttttaaaaaactttatttgtttggtataacagttaggcacgcattttggaaataaaccgatcttttattgggagaagtgccagttaaagtgctggaccaagtaaaatgtttccaaaaaaccagacacgtattaataaaacacttcaaactttttcaaattttacaacaattttcaattaaagttttggcaaattggtaaattttcaagaaatttttttgcaagttttaatttttaaatatacattataattattagaatgttttctgtatagaaaatctctgcatctgttggaaatgaataaaaacttcataacaattctaaattaaaaattcaagaacactagaacaagcgtcctcaaaatattcacaaaatattgtacacattttgtagtactttttccattattaaaaaaagcaaaactagcttttccttcctttccgaaatttaatgctattttcaagatgacttttttgcttgcgtttttctcagtttcctcacacacacacacacacaagtaggcgtggcctgtggaacgtttcagagcgcagaacacctgcatttgatctattcacttcttgcttttgaaaagcccaaagacaccctacacttcggtttcgttttggaaaccattgacatcatcctattttccataagaagtttccttgagaagaatccatttcgcaaatttttcattaaaacgttcaaaactcatcaaaccatttgtaaatagtaataaagtatgtcctgcggctatttgctttctcttcggaatctacaacgccccctcctaatacatcgtttcaggtataaaaagactgcgcctagccgctcgtctcactttttgcagttcatactgaataaaaaaGTACCGCTCGTGGTGCCTATGGTAGCGGAGCaaatcatatgtttttctctctcacactctcttttttcatactctctcttgctgtctagaatttgattggtgtcgcttaacccccctttccctccgaaggaaagttatctccccagatctcttttggtgttttttatcagctaacaacacacattttctgatatttctatgctctgtctatgaacaataaaggcgttgttaattactcgcaaaatcactttgtttatttttttcacattttcagatagtgaacaaaagaaaattaaattctaaaatctgaatcggaaaattcaaattaaaaattaaatttattttttttatattacacctgttttttttcaaatattagatcaaaaactattcaacaagtggcatgtaaagcataacgaggtatatgggcttcagatcttcaact
>syb2493:
cactttttgcagttcatactgaataaaaaaATGGTCTCCCGTCGTCCATGGCTCCTCGCCCTCGCCCTCGCCGTCGCCCTCGCCGCCGGATCCGCCGGAGCCTCCACCGGATCCGACGCCACCGTCCCAGTCGCCACCCAAGACGGACCAGACTACGTCTTCCACCGTGCCCACGAGCGTATGCTCTTCCAAACCTCCTACACCCTCGAGAACAACGGATCCGTCATCTGCATCCCAAACAACGGACAATGCTTCTGCCTCGCCTGGCTCAAGTCCAACGGAACCAACGCCGAGAAGCTCGCCGCCAACATCCTCCAATGGGTCGTCTTCGCCCTCTCCGTCGCCTGCCTCGGATGGTACGCCTACCAAGCCTGGCGTGCCACCTGCGGATGGGAGGAGGTCTACGTCGCCCTCATCGAGATGATGAAGTCCATCATCGAGGCCTTCCACGAGTTCGACTCCCCAGCCACCCTCTGGCTCTCCTCCGGAAACGGAGTCGTCTGGATGCGTTACGGAGAGTGGCTCCTCACCTGCCCAGTCATCCTCATCCACCTCTCCAACCTCACCGGACTCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGACGACTACTCCAAGCGTACCATGGGACTCCTCGTCTCCGACGTCGGATGCATCGTCTGGGGAGCCACCTCCGCCATGTGCACCGGATGGACCAAGATCCTCTTCTTCCTCATCTCCCTCTCCTACGGAATGTACACCTACTTCCACGCCGCCAAGGTCTACATCGAGGCCTTCCACACCGTCCCAAAGGGACTCTGCCGTCAACTCGTCCGTGCCATGGCCTGGCTCTTCTTCGTCTCCTGGGGAATGTTCCCAGTCCTCTTCCTCCTCGGACCAGAGGGATTCGGACACATCTCCCCATACGGATCCGCCATCGGACACTCCATCCTCGACCTCATCGCCAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagAACATGTGGGGAGTCCTCGGAAACTACCTCCGTGTCAAGATCCACGAGCACATCCTCCTCTACGGAGACATCCGTAAGAAGCAAAAGATCACCATCGCCGGACAAGAGATGGAGGTCGAGACCCTCGTCGCCGAGGAGGAGGACAAGTACGAGTCCTCCGGAGGATCCGGAGGAGGATCCGGAGGAATGTCCGAGCTCATCAAGGAGAACATGCACATGAAGCTCTACATGGAGGGAACCGTCAACAACCACCACTTCAAGTGCACCTCCGAGGGAGAGGGAAAGCCATACGAGGGAACCCAAACCATGCGTATCAAGGCCGTCGAGGGAGGACCACTCCCATTCGCCTTCGACATCCTCGCCACCTCCTTCATGTACGGATCCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCTTCATCAACCACACCCAAGGAATCCCAGACTTCTTCAAGCAATCCTTCCCAGAGGGATTCACCTGGGAGCGTGTCACCACCTACGAGGACGGAGGAGTCCTCACCGCCACCCAAGACACCTCCCTCCAAGACGGATGCCTCATCTACAACGTCAAGATCCGTGGAGTCAACTTCCCATCCAACGGACCAGTCATGCAAAAGAAGACCCTCGGATGGGAGGCCTCCACCGAGACCCTCTACCCAGCCGACGGAGGACTCGAGGGACGTGCCGACATGGCCCTCAAGCTCGTCGGAGGAGGACACCTCATCTGCAACCTCAAGACCACCTACCGTTCCAAGAAGCCAGCCAAGAACCTCAAGATGCCAGGAGTCTACTACGTCGACCGTCGTCTCGAGCGTATCAAGGAGGCCGACAAGGAGACCTACGTCGAGCAACACGAGGTCGCCGTCGCCCGTTACTGCGACCTCCCATCCAAGCTCGGACACCGTTAAaaatcatatgtttttctctctcacactctc |
| Phenotype |