| Strain Name | PHX1260 |
| Genotype | aspm-1(syb1260)klp-15(ok1958)klp-16(or1952)/hT2[bli-4(e937) let-?(q782) qIs48] |
| Description | syb1260 is a GFP knock-in inserted before the initiation codon of endogenous aspm-1 gene on klp-15(ok1958) klp-16(or1952) background strain by CRISPR/Cas9 system.The allele is balanced by hT2[bli-4(e937) let-?(q782) qIs48]. Heterozygotes are wild-type worms with pharynx GFP and segregate wild type worms with pharynx GFP. Pick wild-type GFP+ to maintain the strain.
|
| Wild type Sequence | >Wild type
tttattttttgtatttttcagaatcaaa*ATGGATAATAACGTTGAGGATAAAAAAGAGGCAAATCGACACGgtaacaagatatttatactttatctcattatttattttcttcctctttcagAGAAA |
| Mutant Sequence |
>syb1260
tttattttttgtatttttcagaatcaaaATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAATGGATAATAACGTTGAGGATAAAAAAGAGGCAATGCTCAAACGATTGGCAAATCGACACGgtaacaagatatttatactttatctcattatttattttcttcctctttcagAGAAG |
| Phenotype |