| Strain Name | PHX2685 |
| Genotype | ins-6(syb2685) |
| Description | syb2685 is a T2A::3XNLS::GFP knock-in inserted before the termination codon of endogenous ins-6 gene on N2 by CRISPR/Cas9 system.
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| Wild type Sequence | >Wild type
CCACgtaagttgaattaaatgtttacataattcaattatttatttcttgaactttcagAAGAAGGAAAGGACATTGCGACTGAATGCTGCGGAAATCAGTGTTCTGATGACTACATAAGATCTGCTTGTTGTCCA*TGAatttttacctttactccgatatcagtc |
| Mutant Sequence |
>syb2685:
CCtCgtaagttgaattaaatgtttacataattcaattatttatttcttgaactttcagAAGAAGGAAAGGACATTGCGACTGAATGTTGCGGAAATCAGTGTTCTGATGACTACATAAGATCTGCTTGTTGTCCAgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaACCGCTCCAAAGAAGAAACGCAAAGTACCGAGCTCAGAAAAAATGACGGCACCTAAAAAGAAACGCAAAGTACCGGTCGCCGAGAAGACAGCCCCAAAAAAGAAGCGTAAAGTTCCACGCGTCATGGGAgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagCGCGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGTGAatttttacctttactccgatatcagtc |
| Phenotype |