| Strain Name | PHX2704 |
| Genotype | nlp-50(syb2704) |
| Description | syb2704 is a T2A::3XNLS::GFP knock-in inserted before the termination codon of endogenous nlp-50 gene on N2 by CRISPR/Cas9 system.
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| Wild type Sequence | >Wild type
GCCAGTGCAAATGCTTATTACAGACTTGGA*TAGagcagctcacttttgtttcacaatttg |
| Mutant Sequence |
>syb2704:
GCAAGTGCAAATGCTTATTACAGACTTGGAgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaACCGCTCCAAAGAAGAAACGCAAAGTACCGAGCTCAGAAAAAATGACGGCACCTAAAAAGAAACGCAAAGTACCGGTCGCCGAGAAGACAGCCCCAAAAAAGAAGCGTAAAGTTCCACGCGTCATGGGAgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagCGCGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGTAGagcagctcacttttgtttcacaatttg |
| Phenotype |