Strain Name | PHX2805 |
Genotype | nlp-51(syb2805) |
Description | syb2805 is a T2A::3XNLS::GFP knock-in inserted before the termination codon of endogenous nlp-51 gene on N2 by CRISPR/Cas9 system.
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Wild type Sequence | >Wild type
CATAACTGGATGTTCCGTCCGGACGTGGCTCCATATTTTGAG*TAGacattttctgatctcaatacacaccaa |
Mutant Sequence |
>syb2805:
CACAACTGGATGTTCCGTCCTGACGTGGCTCCATATTTTGAGgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaACCGCTCCAAAGAAGAAACGCAAAGTACCGAGCTCAGAAAAAATGACGGCACCTAAAAAGAAACGCAAAGTACCGGTCGCCGAGAAGACAGCCCCAAAAAAGAAGCGTAAAGTTCCACGCGTCATGGGAgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagCGCGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGTAGacattttctgatctcaatacacaccaa |
Phenotype |