| Strain Name | PHX2880 |
| Genotype | ceh-16(syb2880 syb2709) |
| Description | syb2880 is a GFP(LoxP) knock-in inserted before the termination codon of endogenous ceh-16 gene on syb2709 strain by CRISPR/Cas9 system..
syb2709 is a LoxP knock-in inserted before the start codon of endogenous ceh-16 gene on N2 by CRISPR/Cas9 system..
|
| Wild type Sequence | >syb2709-Wild type
acttttttttcgaatttaagatgccaagat*aaaggataacaacaagcgctcgccataaaa
>syb2880-Wild type
ACTCCAAATCCTCATAATCATCCGTCAATTCATGGAGGATATCAACTTATGGCTCAGTTGGCAAAGGTTCAGGCTCGAGCTTACATGCCA*TGAaatattgatttttgttaggcggttacg |
| Mutant Sequence |
>syb2709:
acttttttttcgaatttaagatgccaagatATAACTTCGTATAGCATACATTATACGAAGTTATaaaggataacaacaagcgctcgccataaaa
>syb2880:
ACACCAAATCCTCACAATCATCCGTCAATTCATGGAGGATATCAACTTATGGCTCAGTTGGCTAAGGTTCAGGCTCGAGCTTACATGCCAAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaacATAACTTCGTATAGCATACATTATACGAAGTTATtaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATGAaatattgatttttgttaggcggttacg |
| Phenotype |