Strain Name | PHX2934 |
Genotype | ceh-36(syb2934) ceh-37(syb2933) |
Description | syb2933 is a LoxP knock-in inserted 2403bp downstream of the initiation codon of endogenous ceh-37 gene on N2 by CRISPR/Cas9 system..
syb2934 is a GFP(LoxP) knock-in inserted before the termination codon of endogenous ceh-36 gene on syb2933 strain by CRISPR/Cas9 system..
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Wild type Sequence | >syb2933-Wild type
aataggtgtgagcacgatctacgtccactt*tgggagatcaaattagttcattgaacacct
>syb2934-Wild type
ACCCCAAATATTACTGCCTACTCTCCACTT*TAAgttttgaatattcccttttgattttta |
Mutant Sequence |
>syb2933:
aataggtgtgagcacgatctacgtccacttATAACTTCGTATAGCATACATTATACGAAGTTATtgggagatcaaattagttcattgaacacct
>syb2934:
ACCCCAAAcATTACTGCCTACTCTCCACTTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaacATAACTTCGTATAGCATACATTATACGAAGTTATtaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAAgttttgaatattcccttttgattttta |
Phenotype |