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Strain Name
PHX2934
Genotype
ceh-36(syb2934) ceh-37(syb2933)
Description
syb2933 is a LoxP knock-in inserted 2403bp downstream of the initiation codon of endogenous ceh-37 gene on N2 by CRISPR/Cas9 system.. syb2934 is a GFP(LoxP) knock-in inserted before the termination codon of endogenous ceh-36 gene on syb2933 strain by CRISPR/Cas9 system..
Wild type Sequence
>syb2933-Wild type aataggtgtgagcacgatctacgtccactt*tgggagatcaaattagttcattgaacacct >syb2934-Wild type ACCCCAAATATTACTGCCTACTCTCCACTT*TAAgttttgaatattcccttttgattttta
Mutant Sequence
>syb2933: aataggtgtgagcacgatctacgtccacttATAACTTCGTATAGCATACATTATACGAAGTTATtgggagatcaaattagttcattgaacacct >syb2934: ACCCCAAAcATTACTGCCTACTCTCCACTTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaacATAACTTCGTATAGCATACATTATACGAAGTTATtaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAAgttttgaatattcccttttgattttta
Phenotype