| Strain Name | PHX3112 |
| Genotype | nlp-12(syb3112) |
| Description | syb3112 is a T2A::3XNLS::GFP knock-in inserted before the termination codon of endogenous nlp-12 gene on N2 by CRISPR/Cas9 system. |
| Wild type Sequence | >Wild type
CAGTTCGGAAAGCGAAGCAGCGGCAGCTCTGGACCAGTTGTGCTCGAGCCAATCTGGGAATGGCAA*TAAccgaccccgtctaactatttttccctg |
| Mutant Sequence |
>syb3112:
CAATTCGGAAAGCGAAGCAGCGGCAGCTCTGGACCAGTTGTGCTCGAACCAATCTGGGAATGGCAAgagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaACCGCTCCAAAGAAGAAACGCAAAGTACCGAGCTCAGAAAAAATGACGGCACCTAAAAAGAAACGCAAAGTACCGGTCGCCGAGAAGACAGCCCCAAAAAAGAAGCGTAAAGTTCCACGCGTCATGGGAgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagCGCGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTgTTATGGTGTTCAATGCTTcTCgAGATACCCAGATCATATGAAACgGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAAccgaccccgtctaactatttttccctg |
| Phenotype |