| Strain Name | PHX3190 |
| Genotype | lgc-38(syb2346 syb3190) |
| Description | syb2346 is Pflp-11::dpy-10Crispr site::flp-11b-3’utr inserted at the 5'UTR of endogenous lgc-38 gene on N2 strain by CRISPR/Cas9 system..
syb3190 is a unc-58(e665)-linker(GSGSGSGSG)-mKate2 knock-in inserted with dpy-10 Crispr site on syb2346 strain by CRISPR/Cas9 system..
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| Wild type Sequence | >syb2346-Wild type
gttaaataaatgaggtatatagttaaataa*acgaggtatatgggcttcagatcttcaact
>syb3190-Wild type
cactttttgcagttcatactgaataaaaaaGTACCGCTCGTGGTGCCTATGGTAGCGGAGCaaatcatatgtttttctctctcacactctc |
| Mutant Sequence |
>syb2346:
gttaaataaatgaggtatatagttaaataacacaccagatgatgtgagtgggcctgggtcgagctgcacattttggagaaacccaaaaaccgtgtttcataagtttgaatacaatgactttgtttttttgaaggatttttgtgcagcgttgttttaaaaaacggttttcataagatttttatcacagttgaactcctatatttcttccctcactaattcttgatgtgtggacaaaactgtaagtctgatcaaggcaacaacacaacatagaccacttttctagtccagtaaaaaatcgaactctgatattcagactggtatcacttcttagagtcagaagatggtacagcttgggaaaaacttggaaaaactatcccagactgccaaaactgaagcttaaagtgagcacaaaattgctccaaactttttttgcagctcacattcggtccaagtgttgttcaatttatgatgattatcagtctgttttgccaaattcggatccaactttttttttttttttaggagaatttattttaaagctcgccattgaaatacatgtaaaaatctaagcttttaaatttaacagaaaatgagattatcttttttggaatgaaacctagaattttcaaaataactagcatctcaaaataactagcatccaagatgctctttgttttcataaattttagttgtttccagtcccgaaaaaaaatttcaatcatttccttaggttcaataagtgttttgtctggtctcttgttgcaatttttgaaaaaggaataacattgtgctcagactgaaaaatgaaaaaagatgatgtaagttggtctaggctttaattccagtttccgttccactagtgatcttatcatttattcatatcaagtttcatttgatgttcgattcattgtaggtttcattgttccctctgactattcatcaagttctatgaaaccattgtggcttactagttccccaaagttggaaagatctggagaaagaaaaacaagtatcccaaaagcccaaaccttttttttccaaatgttgagcctctaaagttgatgaatggggtgagagaggttttcatggttttatggcccatcagttccagtagagttcctcatatcttttcgcacttcatttttcaatctgttaagagtaattcccccacaacttttagcatcctcaatcctggttccacccacaaacacccacactccattgaatataagattataaaatataagttaaaatacgaaaagatgcaaaactgtgacgacgaaacttaacaacctgctgacgtgtcaaatatttccgcatcttaccttcgtgggcatcttctaactcataaatttatgatgttcgaatcccggcgccaagtgatgtaaactcatttaatatttgaggaaaaagcatagacgaatccattttgcatctaatttatttttctagtttcacacccatgtttcgcttggcataatcagtgtttgagcgcactttttgcaaggtgcgtcttcttgtaaagaaagattttcaaaaaagtgcaaaagtgggggagattgaaaaacgataagaaggtttcatgtcaaacaaggacacacgaatatataaatacgggtcatttttcaaactttccattgtgactttttggaatatttttttcgtaaaaatgaaatcgattttctgaccaatatctcaactttaaaacttctaattctgctttttttcaatcttctgtaaacaggagtgcgtcgaaatttcggcagtttcggcatattgccaaaagtttatagtccaacaattgccggaactggtgatttagctaaacaataaacctcaaaatattaggtatttcagtaagaaaatgacctattgattatgactttgaatcaagttttgatatatttttaatttttgaaatatgatgaaaattaaaaattatcaaaaattctggcaattttggcacatcggcacacaatgcgaaatttgcggcaactgccgatttttaaaaaactttatttgtttggtataacagttaggcacgcattttggaaataaaccgatcttttattgggagaagtgccagttaaagtgctggaccaagtaaaatgtttccaaaaaaccagacacgtattaataaaacacttcaaactttttcaaattttacaacaattttcaattaaagttttggcaaattggtaaattttcaagaaatttttttgcaagttttaatttttaaatatacattataattattagaatgttttctgtatagaaaatctctgcatctgttggaaatgaataaaaacttcataacaattctaaattaaaaattcaagaacactagaacaagcgtcctcaaaatattcacaaaatattgtacacattttgtagtactttttccattattaaaaaaagcaaaactagcttttccttcctttccgaaatttaatgctattttcaagatgacttttttgcttgcgtttttctcagtttcctcacacacacacacacacaagtaggcgtggcctgtggaacgtttcagagcgcagaacacctgcatttgatctattcacttcttgcttttgaaaagcccaaagacaccctacacttcggtttcgttttggaaaccattgacatcatcctattttccataagaagtttccttgagaagaatccatttcgcaaatttttcattaaaacgttcaaaactcatcaaaccatttgtaaatagtaataaagtatgtcctgcggctatttgctttctcttcggaatctacaacgccccctcctaatacatcgtttcaggtataaaaagactgcgcctagccgctcgtctcactttttgcagttcatactgaataaaaaaGTACCGCTCGTGGTGCCTATGGTAGCGGAGCaaatcatatgtttttctctctcacactctcttttttcatactctctcttgctgtctagaatttgattggtgtcgcttaacccccctttccctccgaaggaaagttatctccccagatctcttttggtgttttttatcagctaacaacacacattttctgatatttctatgctctgtctatgaacaataaaggcgttgttaattactcgcaaaatcactttgtttatttttttcacattttcagatagtgaacaaaagaaaattaaattctaaaatctgaatcggaaaattcaaattaaaaattaaatttattttttttatattacacctgttttttttcaaatattagatcaaaaactattcaacaagtggcatgtaaagcataacgaggtatatgggcttcagatcttcaact
>syb3190:
cactttttgcagttcatactgaataaaaaaATGGCTCCACTGACTGTGAAAAGCTCACCTCCAAAAAAGGCAAAAGGAATATCAAAATTTCGGAGAAAAAAGAAGCAGCCACCACCAGACTCAACCGTATTCGTCGCATGGGCACTCCGAAGTGTCCGAGAGTCTCTGATCCAAGTTGATCCATTGGCCGCTGCACTTGCACATCAGGCTCGAAAGACAAATAGTGTGCCAGCTGTCTCGAGAACTCCACTGCTTCTACAGTTCACTCCTTTCGGACCACCTCTCAGTGCGTATCATGTGACAGCTCGGTGGGAAGGTGCAAATATCAATTCACAATCAGCATTGCTCGATGCAGATGATGGAGCTACAGTTATCACAGATACCATCAAAGATGACCAAGATGATAAAGAACCAAAAAGCTGCCCGCAACAGACTGTCAAATACATCAAAATACTTACACCTCACGTGATCTTGGTGTCAGTGTTAATTGGATATTTATGCTTGGGAGCTTGGATACTCATGTTACTGGAAACAAGGACGGAACTTCTTGCCAGATCCAAAAAACTTGTCAGGTTAACAAATTTGATGTCAAACTTCACTGCCGAAAGTTGGAAGATGCTCAATAATGCTCAACACGGGGTTAGTAATATGGATGAAGGTGAATGGGCTGCAACATTTCGAGAATGGATGGTACGAGTATCAGAAACAGTGGACGATAGGAGACCTATACGACGTGAATTAAACCGGCCTGATGACTTATCAAATATGCATAATAAATGGACATTTCCAACTGCAATATTATATGTTCTCACTGTGTTAACTACTTGCGGTTATGGAGAAGTATCTGTCGACACAGACGTCGGAAAGGTTTTCTCAGTAGCATTCGCGCTTGTTGGTATACCACTTATGTTCATAACAGCTGCCGATATTGGTAAATTTTTATCTGAAACATTACTCCAGTTTGTGAGCTTTTGGAATCGAAGTGTCCGAAAAGTGAAGCAATGGATGAGTCGTATTCGTCACGGCAGGAGAAAGTCATTACAATCAACGGGTGGTCCCAACGATACTCTCGATATTCTTGGTGTCGACGGAACTGAAGAGAAACTTTGGTTCCCAATAGGTGCATATGTATCATGTATTTGCATATATTGCTCAATTGGGTCTGCCATGTTTATCACATGGGAAAGAACTTGGTCTTTCATTCATGCGTTTCATTTTGGTTTCAATTTGATTGTAACAGTCGGACTCGGAGATATCGTTGTGACTGATTACATATTTTTATCACTTATCGTTGCATTTGTGATAGTTGGTTTTTCCGTAGTGACCATGTGCGTGGATCTTGCGTCCACACATCTCAAGGCGTACTTCACCAGAATTCACTACTTTGGTCGAGCAAAACGATTCTTAGGAATGAGTGAGGAACTCAAAGAAATCGTTGCTTTACTGGGGGCGATGCGACGGAAAAAAGGCGGTAAAGTTACATGGAATGATGTGCGAGACTTCCTGGATAACGAACTCCGCGATCGACCTTTTGAACCTCATGAGCTTCTGATGAAGCTCAGATTTATTGACGAAACATCTTCTGGAATGTCTACAATCCGTCACAATTCCTTCCAGTCAGATTTTTTCCGAGAATCAGAGTACATCCGAAGAGTGGCTGCGCTGAGGCCAGAACAGCCAGCATATTTGGGATCCGGATCCGGATCCGGATCCGGAATGTCCGAGCTCATCAAGGAGAACATGCACATGAAGCTCTACATGGAGGGAACCGTCAACAACCACCACTTCAAGTGCACCTCCGAGGGAGAGGGAAAGCCATACGAGGGAACCCAAACCATGCGTATCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagGCCGTCGAGGGAGGACCACTCCCATTCGCCTTCGACATCCTCGCCACCTCCTTCATGTACGGATCCAAGACCTTCATCAACCACACCCAAGGAATCCCAGACTTCTTCAAGCAATCCTTCCCAGAGGGATTCACCTGGGAGCGTGTCACCACCTACGAGGACGGAGGAGTCCTCACCGCCACCCAAGACACCTCCCTCCAAGACGGATGCCTCATCTACAACGTCAAGATCCGTGGAGTCAACTTCCCATCCAACGGACCAGTCATGCAAAAGAAGACCCTCGGATGGGAGGCCTCCACCGAGACCCTCTACCCAGCCGACGGAGGACTCGAGGGACGTGCCGACATGGCCCTCAAGCTCGTCGGAGGAGGACACCTCATCTGCAACCTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCACCTACCGTTCCAAGAAGCCAGCCAAGAACCTCAAGATGCCAGGAGTCTACTACGTCGACCGTCGTCTCGAGCGTATCAAGGAGGCCGACAAGGAGACCTACGTCGAGCAACACGAGGTCGCCGTCGCCCGTTACTGCGACCTCCCATCCAAGCTCGGACACCGTTAAaaatcatatgtttttctctctcacactctc |
| Phenotype |